Publication detail

Comparison of Negative-ion Electrospray Mass Spectra Measured by Seven Tandem Mass Analyzers Towards Library Formation

VOLNÁ, K. HOLČAPEK, M. KOLÁŘOVÁ, L. LEMR, K. ČÁSLAVSKÝ, J. KAČER, P. POUSTKA, J. HUBÁLEK, M.

Original Title

Type

journal article - other

Language

English

Original Abstract

A library of negative ion electrospray ionization mass spectra and tandem mass spectra (MS/MS) of sulfonated dyes has been developed for fast identification purposes. The uniform protocol has been elaborated and applied to the measurements of more than 50 anionic dyes. Three collision energies are selected in our protocol which ensures that at least one of them provides a suitable ratio of product ions to the precursor ion. The robustness is investigated with altered values of tuning parameters (e.g. the pressure of the nebulizing gas, the temperature and the flow rate of drying gas, and the mobile phase composition). The results of the inter-laboratory comparison of product ion mass spectra recorded on seven different tandem mass spectrometers (three ion traps, two triple quadrupoles and two hybrid quadrupole time of flight instruments) are presented for four representative anionic dyes - azo dye Acid Red 118, anthraquinone dye Acid Violet 43, triphenylmethane dye Acid Blue 1 and Al(III) metal-complex azo dye. The fragmentation patterns are almost identical for all tandem mass analyzers, only the ratios of product ions differ somewhat which confirms the possibility of spectra transfer among different mass analyzers with the goal of library formation.

Keywords

chromatography/mass spectrometry instruments; collisionally induced dissociation; liquid-chromatography; sulfonated dyes; azo dyestuffs; ionization; drugs; trap; identification; searching

Authors

VOLNÁ, K.; HOLČAPEK, M.; KOLÁŘOVÁ, L.; LEMR, K.; ČÁSLAVSKÝ, J.; KAČER, P.; POUSTKA, J.; HUBÁLEK, M.

RIV year

2008

Released

30. 1. 2008

Publisher

Wiley

Location

London

ISBN

0951-4198

Periodical

RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Year of study

Number

State

United Kingdom of Great Britain and Northern Ireland

Pages from

101

Pages to

108

Pages count

BibTex

@article{BUT43497,
  author="Kateřina {Volná} and Michal {Holčapek} and Karel {Lemr} and Josef {Čáslavský} and Petr {Kačer} and Jan {Poustka}",
  title="Comparison of Negative-ion Electrospray Mass Spectra Measured by Seven Tandem Mass Analyzers Towards Library Formation",
  journal="RAPID COMMUNICATIONS IN MASS SPECTROMETRY",
  year="2008",
  volume="22",
  number="2",
  pages="101--108",
  issn="0951-4198"
}