Publication detail

Smallest dual-color qPCR device

Hanliang Zhu Xiaocheng Liu Yingqi Wang Antao Sun Tomas Teply Marie Korabecna Haoqing Zhang Pavel Neuzil

Original Title

Smallest dual-color qPCR device

Type

journal article in Web of Science

Language

English

Original Abstract

The global outbreak of the coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), necessitated the development of point-of-care (POC) systems for rapid and widespread population screening. Current POC systems, based on reverse transcription–quantitative polymerase chain reaction (RT-qPCR) methods, are unable to perform RNA target multiplexing, which is crucial for specific viral strain identification. This study presents a compact qPCR system capable of dual fluorescence channel gene multiplexing by simultaneously detecting two targets of the SARS-CoV-2 virus using 6-carboxyfluorescein (FAM)-and TxRed-labeled probes. The device measures (110 × 83 × 41) mm3 and weighs 210 gm. It features four virtual reaction chambers, a thermal cycle system, a real-time optical signal detection module, a USB interface, and a Bluetooth wireless data transmission module. Moreover, it can perform single fluorescent channel detection of PCR using intercalating dye, followed by a melting curve analysis and loop-mediated isothermal amplification. The proposed compact dual-color qPCR device significantly broadens its potential for POC applications, such as diagnostics or field testing, molecular biology, microfluidics, and virus detection.

Keywords

Miniaturization/integration; Multiplexing; RT-qPCR; Virus detection

Authors

Hanliang Zhu; Xiaocheng Liu; Yingqi Wang; Antao Sun; Tomas Teply; Marie Korabecna; Haoqing Zhang; Pavel Neuzil

Released

1. 11. 2023

Publisher

ELSEVIER SCIENCE SA

Location

LAUSANNE

ISBN

0925-4005

Periodical

Sensors and Actuators B: Chemical

Year of study

394

Number

November 2023

State

Swiss Confederation

Pages from

1

Pages to

9

Pages count

9

URL

https://www.sciencedirect.com/science/article/pii/S0925400523010146

BibTex

@article{BUT186765,
  author="Hanliang Zhu and Xiaocheng Liu and Yingqi Wang and Antao Sun and Tomas Teply and Marie Korabecna and Haoqing Zhang and Pavel Neuzil",
  title="Smallest dual-color qPCR device",
  journal="Sensors and Actuators B: Chemical",
  year="2023",
  volume="394",
  number="November 2023",
  pages="1--9",
  doi="10.1016/j.snb.2023.134299",
  issn="0925-4005",
  url="https://www.sciencedirect.com/science/article/pii/S0925400523010146"
}