Publication detail

Enhancing the substrate selectivity of enzyme mimetics in biosensing and bioassay: Novel approaches

ASHRAFI, A. MUKHERJEE, A. SAADATI, A. MATYSIK, F. RICHTERA, L. ADAM, V.

Original Title

Enhancing the substrate selectivity of enzyme mimetics in biosensing and bioassay: Novel approaches

Type

journal article in Web of Science

Language

English

Original Abstract

A substantial development in nanoscale materials possessing catalytic activities comparable with natural enzymes has been accomplished. Their advantages were owing to the excellent sturdiness in an extreme environment, possibilities of their large-scale production resulting in higher profitability, and easy manipulation for modification. Despite these advantages, the main challenge for artificial enzyme mimetics is the lack of substrate selectivity where natural enzymes flourish. This review addresses this vital problem by introducing substrate selectivity strategies to three classes of artificial enzymes: molecularly imprinted polymers, nanozymes (NZs), and DNAzymes. These rationally designed strategies enhance the substrate selectivity and are discussed and exemplified throughout the review. Various functional mechanisms associated with applying enzyme mimetics in biosensing and bioassays are also given. Eventually, future directives toward enhancing the substrate selectivity of biomimetics and related challenges are discussed and evaluated based on their efficiency and convenience in biosensing and bioassays.

Keywords

Nanozymes; Molecularly-imprinted-polymer; DNAzymes; Artificial-enzyme mimetics; Enhanced selectivity

Authors

ASHRAFI, A.; MUKHERJEE, A.; SAADATI, A.; MATYSIK, F.; RICHTERA, L.; ADAM, V.

Released

1. 9. 2024

Publisher

ELSEVIER

Location

AMSTERDAM

ISBN

1873-3727

Periodical

ADVANCES IN COLLOID AND INTERFACE SCIENCE

Year of study

331

Number

103233

State

Kingdom of the Netherlands

Pages count

21

URL

https://www.sciencedirect.com/science/article/pii/S0001868624001568?via%3Dihub

BibTex

@article{BUT190010,
  author="Amirmansoor {Ashrafi} and Atripan {Mukherjee} and Arezoo {Saadati} and Frank-Michael {Matysik} and Lukáš {Richtera} and Vojtěch {Adam}",
  title="Enhancing the substrate selectivity of enzyme mimetics in biosensing and bioassay: Novel approaches",
  journal="ADVANCES IN COLLOID AND INTERFACE SCIENCE",
  year="2024",
  volume="331",
  number="103233",
  pages="21",
  doi="10.1016/j.cis.2024.103233",
  issn="1873-3727",
  url="https://www.sciencedirect.com/science/article/pii/S0001868624001568?via%3Dihub"
}