Publication detail

In vivo evaluation of selenium-tellurium based nanoparticles as a novel treatment for bovine mastitis

KOŠARIŠŤANOVÁ, L. BYTEŠNÍKOVÁ, Z. FIALOVÁ, T. PEKÁRKOVÁ, J. ŠVEC, P. ONDREÁŠ, F. JEMELÍKOVÁ, V. RIDOŠKOVÁ, A. MAKOVICKÝ, P. SIVÁK, L. DOLEJSKÁ, M. ZOUHAROVÁ, M. SLÁMA, P. ADAM, V. ŠMERKOVÁ, K.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

Background Bovine mastitis is one of the main causes of reduced production in dairy cows. The infection of the mammary gland is mainly caused by the bacterium Staphylococcus aureus, whose resistant strains make the treatment of mastitis with conventional antibiotics very difficult and result in high losses. Therefore, it is important to develop novel therapeutic agents to overcome the resistance of mastitis-causing strains. In this study, novel selenium-tellurium based nanoparticles (SeTeNPs) were synthesized and characterized. Their antibacterial activity and biocompatibility were evaluated both in vitro and in vivo using a bovine model. A total of 10 heifers were divided into experimental and control groups (5 animals each). After intramammary infection with methicillin resistant S. aureus (MRSA) and the development of clinical signs of mastitis, a dose of SeTeNPs was administered to all quarters in the experimental group. Results Based on in vitro tests, the concentration of 149.70 mg/L and 263.95 mg/L of Se and Te, respectively, was used for application into the mammary gland. Three days after SeTeNPs administration, MRSA counts in the experimental group showed a significant reduction (P < 0.01) compared to the control group. The inhibitory effect observed within the in vitro experiments was thus confirmed, resulting in the suppression of infection in animals. Moreover, the superior biocompatibility of SeTeNPs in the organism was demonstrated, as the nanoparticles did not significantly alter the inflammatory response or histopathology at the site of application, i.e., mammary gland, compared to the control group (P > 0.05). Additionally, the metabolic profile of the blood plasma as well as the histology of the main organs remained unaffected, indicating that the nanoparticles had no adverse effects on the organism. Conclusions Our findings suggest that SeTeNPs can be used as a promising treatment for bovine mastitis in the presence of resistant bacteria. However, the current study is limited by its small sample size, making it primarily a proof of the concept for the efficacy of intramammary-applied SeTeNPs. Therefore, further research with a larger sample size is needed to validate these results.

Keywords

Antibacterial; Biocompatibility; Heifer; Intramammary; Mammary gland; MRSA; Nanomaterial; Resistance; SeTe

Authors

KOŠARIŠŤANOVÁ, L.; BYTEŠNÍKOVÁ, Z.; FIALOVÁ, T.; PEKÁRKOVÁ, J.; ŠVEC, P.; ONDREÁŠ, F.; JEMELÍKOVÁ, V.; RIDOŠKOVÁ, A.; MAKOVICKÝ, P.; SIVÁK, L.; DOLEJSKÁ, M.; ZOUHAROVÁ, M.; SLÁMA, P.; ADAM, V.; ŠMERKOVÁ, K.

Released

20. 12. 2024

Publisher

BMC

Location

LONDON

ISBN

2049-1891

Periodical

Journal of Animal Science and Biotechnology

Year of study

Number

State

People's Republic of China

Pages from

Pages to

Pages count

URL

https://jasbsci.biomedcentral.com/articles/10.1186/s40104-024-01128-y

BibTex

@article{BUT193993,
  author="Ludmila {Košarišťanová} and Zuzana {Bytešníková} and Tatiana {Fialová} and Jana {Pekárková} and Pavel {Švec} and František {Ondreáš} and Vendula {Jemelíková} and Andrea {Ridošková} and Peter {Makovický} and Ladislav {Sivák} and Monika {Dolejská} and Monika {Zouharová} and Petr {Sláma} and Vojtěch {Adam} and Kristýna {Šmerková}",
  title="In vivo evaluation of selenium-tellurium based nanoparticles as a novel treatment for bovine mastitis",
  journal="Journal of Animal Science and Biotechnology",
  year="2024",
  volume="15",
  number="1",
  pages="17",
  doi="10.1186/s40104-024-01128-y",
  issn="2049-1891",
  url="https://jasbsci.biomedcentral.com/articles/10.1186/s40104-024-01128-y"
}