Publication detail

YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles

CASSANI, M. FERNANDES, S. CRUZ, J. ĎURÍKOVA, H. VRBSKÝ, J. PATOČKA, M. HEGROVÁ, V. KLIMOVIČ, S. PŘIBYL, J. DEBELLIS, D. SKLÁDAL, P. CAVALIERI, F. CARUSO, F. FORTE, G.

Original Title

YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles

Type

journal article in Web of Science

Language

English

Original Abstract

Interactions between living cells and nanoparticles are extensively studied to enhance the delivery of therapeutics. Nanoparticles size, shape, stiffness, and surface charge are regarded as the main features able to control the fate of cell-nanoparticle interactions. However, the clinical translation of nanotherapies has so far been limited, and there is a need to better understand the biology of cell-nanoparticle interactions. This study investigates the role of cellular mechanosensitive components in cell-nanoparticle interactions. It is demonstrated that the genetic and pharmacologic inhibition of yes-associated protein (YAP), a key component of cancer cell mechanosensing apparatus and Hippo pathway effector, improves nanoparticle internalization in triple-negative breast cancer cells regardless of nanoparticle properties or substrate characteristics. This process occurs through YAP-dependent regulation of endocytic pathways, cell mechanics, and membrane organization. Hence, the study proposes targeting YAP may sensitize triple-negative breast cancer cells to chemotherapy and increase the selectivity of nanotherapy. The inhibition of Yeas-associated protein (YAP) in TNBC cells affects the organization of plasma membrane, reduces the extracellular matrix (ECM) deposition, impacts their adhesion ability, and increases the endocytosis rate. Thus, targeting cell mechanobiology may be leveraged to optimize cell-nanoparticle interactions. Ultimately, these changes contribute cooperatively to promote the delivery of nanomedicines to cancer cells and improve the therapeutic efficiency.image

Keywords

bio-nano interactions; cancer treatment; mechanobiology; nanoparticles; YAP-signaling

Authors

CASSANI, M.; FERNANDES, S.; CRUZ, J.; ĎURÍKOVA, H.; VRBSKÝ, J.; PATOČKA, M.; HEGROVÁ, V.; KLIMOVIČ, S.; PŘIBYL, J.; DEBELLIS, D.; SKLÁDAL, P.; CAVALIERI, F.; CARUSO, F.; FORTE, G.

Released

12. 1. 2024

Publisher

WILEY

Location

HOBOKEN

ISBN

2198-3844

Periodical

Advanced Science

Year of study

11

Number

2

State

United States of America

Pages count

16

URL

https://advanced.onlinelibrary.wiley.com/doi/full/10.1002/advs.202302965

BibTex

@article{BUT197240,
  author="CASSANI, M. and FERNANDES, S. and CRUZ, J. and ĎURÍKOVA, H. and VRBSKÝ, J. and PATOČKA, M. and HEGROVÁ, V. and KLIMOVIČ, S. and PŘIBYL, J. and DEBELLIS, D. and SKLÁDAL, P. and CAVALIERI, F. and CARUSO, F. and FORTE, G.",
  title="YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles",
  journal="Advanced Science",
  year="2024",
  volume="11",
  number="2",
  pages="16",
  doi="10.1002/advs.202302965",
  issn="2198-3844",
  url="https://advanced.onlinelibrary.wiley.com/doi/full/10.1002/advs.202302965"
}