Publication detail
Impact of Genetic Deletions on the Biotechnological Potential and Stress Robustness of Caldimonas thermodepolymerans in PHA Production
ŠLOSÁROVÁ, K. IEREMENKO, A. BUCHTÍKOVÁ, I. PACASOVÁ, V. BERGER, M. DVOŘÁK, P. OBRUČA, S.
Original Title
Impact of Genetic Deletions on the Biotechnological Potential and Stress Robustness of Caldimonas thermodepolymerans in PHA Production
Type
abstract
Language
English
Original Abstract
Bacterial polyhydroxyalkanoates (PHA) represent an interesting source of natural biodegradable substances that are finding applications in an increasing number of industrial fields. They have attracted attention mainly due to their properties similar to petrochemical plastics. Promising producers are thermophilic bacteria, which form the NGIB concept. One adept is the gram-negative bacterium Caldimonas thermodepolymerans DSM 15344, which is capable of producing poly3-hydroxybutyrate (PHB) up to 87% w/w of CDM. To enhance the biotechnological potential of this bacterium, genetic modification techniques have been employed, specifically in the form of deletions based on homologous recombination. In this work, we present a comparison of the biotechnological characteristics and stress robustness in three mutant strains of C. thermodepolymerans DSM15344 together with wild type itself. The genetically modified bacteria involved are: C. thermodepolymerans ΔphaC (derivative of DSM 15344 with deleted gene of PHA synthase IS481_08630); C. thermodepolymerans AI01 (derivative of DSM 15344, deleted genes of restriction endonucleases: ∆IS481_08585, ∆IS481_14855, ∆IS4 81_14025); and C. thermodepolymerans ΔphaZ (derivative of C. thermodepolymerans AI01, deleted gene of PHA intracellular depolymerase IS481_07130). Under the guise of the biological consequences of gene deletions, we focused on the growth of bacteria on three different substrates – xylose, glucose and cellobiose (72 h at 50 °C) in terms of PHA production (except C. thermodepolymerans ΔphaC); stress robustness characterization of the mutants as compared to DSM 15344 wild type; and also comparison of molecular weights of synthesized PHB in ΔphaZ as compared to DSM 15344 wild type.
Keywords
thermophilic bacteria, polyhydroxyalkanoates, genetic modification, homologous recombination
Authors
ŠLOSÁROVÁ, K.; IEREMENKO, A.; BUCHTÍKOVÁ, I.; PACASOVÁ, V.; BERGER, M.; DVOŘÁK, P.; OBRUČA, S.
Released
14. 4. 2025
ISBN
ISBN 978-80-88307-24
Book
book of abstracts
Edition
1
Edition number
1
Pages from
159
Pages to
159
Pages count
1
BibTex
@misc{BUT198379,
author="ŠLOSÁROVÁ, K. and IEREMENKO, A. and BUCHTÍKOVÁ, I. and PACASOVÁ, V. and BERGER, M. and DVOŘÁK, P. and OBRUČA, S.",
title="Impact of Genetic Deletions on the Biotechnological Potential and Stress Robustness of Caldimonas thermodepolymerans in PHA Production",
booktitle="book of abstracts",
year="2025",
series="1",
edition="1",
pages="159--159",
isbn="ISBN 978-80-88307-24",
note="abstract"
}