Publication detail

Optimizing printability and mechanical properties of poly(3-hydroxybutyrate) biocomposite blends and their biological response to Saos-2 cells

KROBOT, Š. MENČÍK, P. CHALOUPKOVÁ, K. BOČKAJ, J. AGÓCSOVÁ, S. KLUSÁČEK RAMPICHOVÁ, M. HEDVIČÁKOVÁ, V. ALEXY, P. PŘIKRYL, R. MELČOVÁ, V.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

Bone tissue engineering requires scaffolds with three-dimensional (3D) structures that facilitate vascularization and new tissue growth. 3D printing, especially through fused deposition modeling (FDM), has emerged as an effective method for creating complex structures with high reproducibility. Early research in this area demonstrated the potential of poly(ε-caprolactone) (PCL) and poly(L-lactide) (PLLA) scaffolds for bone regeneration. Recently, polylactide (PLA) and polyhydroxyalkanoates (PHAs) have garnered attention for their biocompatibility and ability to support cell proliferation. Among PHAs, poly(3-hydroxybutyrate) (P3HB) shows promise due to its intrinsic biocompatibility and resorbability, making it a candidate for FDM-based scaffold fabrication. In the presented study, we aim to develop and optimize a biocompatible P3HB-based composite material for bone tissue engineering, incorporating PLA, hydroxyapatite (HA), and the plasticizer Syncroflex3114 (SN) to enhance mechanical properties and printability. This composite was processed into filaments for 3D printing and characterized through thermal, mechanical, and biological evaluations. Using a design of experiment (DoE) approach, we investigated factors such as temperature performance, warping, degradation, and strength to determine the optimal composition for use in tissue engineering. Four optimal mixture compositions fulfilling the optimization criteria of having the most suitable properties for bone tissue engineering, namely the best printability and maximum mechanical properties, were obtained. The mixtures were optimized specifically for minimum warping coefficient (0.5); maximum flexural strength (66.9 MPa); maximum compression modulus (2.4 GPa); and maximum compression modulus (2.3 GPa) with a warping coefficient of no more than 1 at the same time. In conclusion, the study shows a new possible way to effectively develop and test 3D-printed P3HB-based scaffolds with specifically optimized material properties.

Keywords

Poly(3-hydroxybutyrate); Polylactide; 3D printing; Fused deposition modeling; Design of Experiment; Scaffold; Printability

Authors

KROBOT, Š.; MENČÍK, P.; CHALOUPKOVÁ, K.; BOČKAJ, J.; AGÓCSOVÁ, S.; KLUSÁČEK RAMPICHOVÁ, M.; HEDVIČÁKOVÁ, V.; ALEXY, P.; PŘIKRYL, R.; MELČOVÁ, V.

Released

18. 12. 2024

Publisher

AccScience Publishing

Location

Singapur

ISBN

2424-8002

Periodical

International Journal of Bioprinting

Year of study

Number

State

Republic of Singapore

Pages from

Pages to

Pages count

URL

https://www.accscience.com/journal/IJB/articles/online_first/4158

Full text in the Digital Library

http://hdl.handle.net/11012/251590

BibTex

@article{BUT193562,
  author="Štěpán {Krobot} and Přemysl {Menčík} and Kateřina {Chaloupková} and Ján {Bočkaj} and Sára Vach {Agócsová} and Michala {Klusáček Rampichová} and Věra {Hedvičáková} and Pavol {Alexy} and Radek {Přikryl} and Veronika {Melčová}",
  title="Optimizing printability and mechanical properties of poly(3-hydroxybutyrate) biocomposite blends and their biological response to Saos-2 cells",
  journal="International Journal of Bioprinting",
  year="2024",
  volume="11",
  number="1",
  pages="1--18",
  doi="10.36922/ijb.5175",
  issn="2424-8002",
  url="https://www.accscience.com/journal/IJB/articles/online_first/4158"
}