Publication detail

Effect of enriched lignocellulosic wastes as a primary nutritional source on the biomass quality of selected yeast strains

OBRAČAJ, J. HOLUB, J. ČARNOKÁ, D. ŠPAČKOVÁ, D. GERSPITZEROVÁ, N. MIKULÍKOVÁ, R. MÁROVÁ, I.

Original Title

Type

abstract

Language

English

Original Abstract

Yeasts are a diverse group of microorganisms capable of producing a variety of nutritionally and biologically valuable metabolites, including a wide range of antioxidants such as carotenoids, ubiquinone, ergosterol, β-glucans, microbial lipids and others. One significant benefit of cultivating these microscopic fungi is their ability to utilize various nutrient sources, such as industrial by-products, after appropriate pretreatment of this alternative source. The aim of this work is to optimize the cultivation conditions of selected yeasts on low-cost lignocellulosic waste materials, with the addition of microbiogenic elements (iodine, iron). The goal is to determine which form of mineral is optimally utilized by microorganisms, whether it is possible to fortify the biomass with multiple elements simultaneously, and how much of each element accumulates in the biomass when combined with the selected waste material. The strain Metchnikowia pulcherrima and the strain Rhodotorula toruloides were cultivated under controlled C/N ratios to ensure comparability of the results. Metabolites contained in biomas, were analyzed by various laboratory techniques, included gas chromatography (GC) and high performance liquid chromatography (HPLC). According to the results of the study so far, we can confirm that both strains utilize the waste nutrient sources. The highest biomass yield was recorded in the R. toruloides strain when hydrolyzed brewery spent grain was used in combination with sodium iodate (13.15 mg biomass/ml medium). In the M. pulcherrima strain, the highest biomass yield was recorded when coffee hydrolysate was used as a nutrient source and ferric chloride was added (9.5 mg biomass/ml medium). The results of this work can provide valuable informations with application in industrial biotechnology of enriched yeasts biomass and reduce their economic demandingness and at the time provide aid with ecological disposal of selected waste materials.

Keywords

Yeast, lignocellulosic waste, spent coffee grounds, brewers` spent grain, biogenic elements, Rhodotorula, Metschnikowia, carotenoids, coenzyme Q, fatty acids

Authors

OBRAČAJ, J.; HOLUB, J.; ČARNOKÁ, D.; ŠPAČKOVÁ, D.; GERSPITZEROVÁ, N.; MIKULÍKOVÁ, R.; MÁROVÁ, I.

Released

15. 4. 2025

Publisher

AMCA, spol. s r.o.

Location

Vyšehradská 320/49, 128 00 Prague 2, Czech Republic

ISBN

978-80-88307-24-2

Book

Book of abstracts of the 12th International Conference on Chemical Technology

Edition

1st edition, April 2025

Pages from

154

Pages to

155

Pages count

URL

https://www.icct.cz/cs/Amca-ICCT/media/content/2025/Program/Book_of_abstracts_-ICCT2025.pdf

BibTex

@misc{BUT197836,
  author="Jan {Obračaj} and Jiří {Holub} and Daniela {Čarnoká} and Dominika {Špačková} and Nela {Gerspitzerová} and Renata {Mikulíková} and Ivana {Márová}",
  title="Effect of enriched lignocellulosic wastes as a primary nutritional source on the biomass quality of selected yeast strains",
  booktitle="Book of abstracts of the 12th International Conference on Chemical Technology",
  year="2025",
  series="1st edition, April 2025",
  pages="154--155",
  publisher="AMCA, spol. s r.o.",
  address="Vyšehradská 320/49, 128 00 Prague 2, Czech Republic",
  isbn="978-80-88307-24-2",
  url="https://www.icct.cz/cs/Amca-ICCT/media/content/2025/Program/Book_of_abstracts_-ICCT2025.pdf",
  note="abstract"
}