Publication detail

INNOVATIVE BIOFERTILIZER FORMULATION UTILIZING SELF-GELATING AZOTOBACTER VINELANDII

SÚKENÍK, M. ČERNAYOVÁ, D. KALINA, M. HLAVÁČKOVÁ, B. SEDLÁČEK, P. OBRUČA, S.

Original Title

Type

abstract

Language

English

Original Abstract

The demand for sustainable agriculture necessitates innovative methods to maintain yields while minimizing conventional fertiliser use, thereby enhancing environmental sustainability and protecting fertile land. One such innovation is the development of biofertilizers that utilize plant growth-promoting rhizobacteria (PGPR) to enhance soil quality through various beneficial mechanisms. We have proposed a novel biofertilizer production method employing Azotobacter vinelandii for in-situ self-encapsulation within a protective gel carrier through alginate crosslinking during bacterium culture. This approach streamlines preparation, potentially lowering costs and enhancing process competitiveness. Strains of A. vinelandii were selected for bioinoculant preparation and characterization. Among them, Azotobacter vinelandii CCM 289 was chosen for its superior alginate production, effective gelation, and confirmed ability to generate PGP metabolites, such as indole-3-acetic acid and siderophores. To confirm the positive effect on plant growth, three pot-cultivation experiments were executed on Lactuca sativa L., incorporating four PGPR carrier formulations, with each group comprising 8 plants. These experiments demonstrated positive effects of these formulations mainly under environmental stresses, including low irrigation and nutrient deficiency. Lyophilized gel formulations significantly improved soil microbial diversity, plant growth under stress and plant dry mass. These results highlight the potential of alginate-based PGPR formulations for sustainable agriculture.

Keywords

Azotobacter vinelandii; polyhydroxyalkanoates; alginate; Lactuca sativa

Authors

SÚKENÍK, M.; ČERNAYOVÁ, D.; KALINA, M.; HLAVÁČKOVÁ, B.; SEDLÁČEK, P.; OBRUČA, S.

Released

28. 4. 2025

Publisher

Česká společnost chemická

Location

Praha

ISBN

2336-7210

Book

XIVth INTERDISCIPLINARY MEETING OF YOUNG LIFE SCIENTISTS

Edition

Czech Chemical Society Symposium Series

Edition number

Vol 23 No 2 (2025)

ISBN

2336-7210

Periodical

Czech Chemical Society Symposium Series

Year of study

23 (2025)

Number

State

Czech Republic

Pages from

Pages to

Pages count

URL

http://www.ccsss.cz/index.php/ccsss/issue/view/50/90

BibTex

@misc{BUT197954,
  author="Martin {Súkeník} and Diana {Černayová} and Michal {Kalina} and Barbora {Hlaváčková} and Petr {Sedláček} and Stanislav {Obruča}",
  title="INNOVATIVE BIOFERTILIZER FORMULATION UTILIZING SELF-GELATING AZOTOBACTER VINELANDII",
  booktitle="XIVth INTERDISCIPLINARY MEETING OF YOUNG LIFE SCIENTISTS",
  year="2025",
  series="Czech Chemical Society Symposium Series",
  edition="Vol 23 No 2 (2025)",
  journal="Czech Chemical Society Symposium Series",
  volume="23 (2025)",
  number="2",
  pages="62--62",
  publisher="Česká společnost chemická",
  address="Praha",
  isbn="2336-7210",
  issn="2336-7210",
  url="http://www.ccsss.cz/index.php/ccsss/issue/view/50/90",
  note="abstract"
}