Publication detail

Biotransformation of ferulic acid into valuable products employing halophilic bacterium Halomonas neptunia

HRABALOVÁ, V. ČERNAYOVÁ, D. NÁBĚLEK, J. MATĚJKA, F. POŘÍZKA, J. DIVIŠ, P. OBRUČA, S.

Original Title

Biotransformation of ferulic acid into valuable products employing halophilic bacterium Halomonas neptunia

Type

journal article in Web of Science

Language

English

Original Abstract

This study investigates the potential of the halophilic bacterium Halomonas neptunia for the biotransformation of ferulic acid (FA) into valuable aromatic compounds, with a focus on vanillic acid (VA). H. neptunia demonstrated high efficiency in FA conversion, producing VA as the predominant metabolite alongside minor amounts of vanillin, vanillyl alcohol, and 4-vinylguaiacol. A key advantage of H. neptunia is its halotolerance, enabling biotransformation at NaCl concentrations up to 10 wt. %, which significantly reduces contamination risks from common mesophilic microflora and facilitates non-sterile process operation. H. neptunia cells were encapsulated in alginate-based hydrogel beads using a semi-automated encapsulation system to enhance the efficiency and reusability of the bacterial culture. Encapsulation markedly improved the transformation efficiency of FA (initial concetration of 500 mg/L), with VA concentrations reaching 370 mg/L and conversion rate 85.5 mol. % after 72 h in the first cycle. In subsequent cycles, the initial FA concentration was increased to 460 mg/L. Nearly complete conversion of FA to VA was observed, suggesting a highly positive effect of adaptation of the bacterial culture to the presence of FA. The process demonstrated stability across multiple cycles, supporting its potential for semicontinuous or continuous operation. Additionally, crude FA extracted from wheat bran was successfully used as a substrate without requiring extensive purification, further enhancing the process's sustainability. These findings highlight H. neptunia as a promising candidate for industrial-scale FA biotransformation, offering a robust, low-contamination, and scalable approach to producing high-value aromatic compounds.

Keywords

Halomonas neptunia; Halophiles; Ferulic acid; Biotransformation; Vanillic acid; Encapsulation

Authors

HRABALOVÁ, V.; ČERNAYOVÁ, D.; NÁBĚLEK, J.; MATĚJKA, F.; POŘÍZKA, J.; DIVIŠ, P.; OBRUČA, S.

Released

2. 6. 2025

Publisher

ELSEVIER

Location

AMSTERDAM

ISBN

1878-8181

Periodical

Biocatalysis and Agricultural Biotechnology

Year of study

66

Number

6

State

Kingdom of the Netherlands

Pages from

1

Pages to

10

Pages count

10

URL

https://www.sciencedirect.com/science/article/pii/S1878818125000994?via%3Dihub

BibTex

@article{BUT197971,
  author="Vendula {Hrabalová} and Diana {Černayová} and Jakub {Nábělek} and Filip {Matějka} and Jaromír {Pořízka} and Pavel {Diviš} and Stanislav {Obruča}",
  title="Biotransformation of ferulic acid into valuable products employing halophilic bacterium Halomonas neptunia",
  journal="Biocatalysis and Agricultural Biotechnology",
  year="2025",
  volume="66",
  number="6",
  pages="1--10",
  doi="10.1016/j.bcab.2025.103586",
  issn="1878-8181",
  url="https://www.sciencedirect.com/science/article/pii/S1878818125000994?via%3Dihub"
}