Publication detail

In vitro co-culture study on injectable calcium phosphate cements: investigation of early cell seeding after hardening

KLUSÁČEK RAMPICHOVÁ, M. ŠEBOVÁ, E. VOJTOVÁ, L. MICHLOVSKÁ, L. FILOVA, E.

Original Title

Type

abstract

Language

English

Original Abstract

Calcium phosphate cements (CPCs) are widely explored for bone regeneration due to their osteoconductivity and injectability. However, ion imbalance during cement hardening can create unfavorable conditions for cellular viability and function. This imbalance, primarily involving fluctuations in calcium and phosphate ion concentrations, is usually well tolerated in vivo due to the complexity of the physiological processes. On the other hand, problems may arise in in vitro assays, where we are limited by the buffering capacity of the culture medium. To mitigate this issue and enhance physiological relevance, this study investigated the effect of early cell seeding on CPCs. In addition, we wanted to get closer to physiological conditions by using osteoblast-like Saos-2 cells in coculture with endothelial HUVEC cells, which are involved in bone tissue regeneration and interact with each other. We evaluated cell survival, scaffold biocompatibility, and gene expression profiles.

Keywords

calcium phosphate cement, osteoconductivity, injectability, hardening, Saos-2 celss, HUVEC cells

Authors

KLUSÁČEK RAMPICHOVÁ, M.; ŠEBOVÁ, E.; VOJTOVÁ, L.; MICHLOVSKÁ, L.; FILOVA, E.

Released

23. 4. 2025

Location

Praha

ISBN

978-80-907779-4-1

Book

XVI. mezinárodní konference Bioimplantologie a regenerativní medicína

Edition number

Pages from

Pages to

Pages count

BibTex

@misc{BUT198348,
  author="Michala {Klusáček Rampichová} and Eva {Šebová} and Lucy {Vojtová} and Lenka {Michlovská} and Eva {Filova}",
  title="In vitro co-culture study on injectable calcium phosphate cements: investigation of early cell seeding after hardening",
  booktitle="XVI. mezinárodní konference Bioimplantologie a regenerativní medicína",
  year="2025",
  edition="1",
  pages="53--53",
  address="Praha",
  isbn="978-80-907779-4-1",
  note="abstract"
}