Publication detail

PLGA-PEG-PLGA Core-Shell Nanoparticles for Antibacterial Enzyme Delivery: A Promising Approach for Enhancing Calcium Phosphate Cements

KREUZEROVÁ, M. TUŠLOVÁ, K. KADLECOVÁ, Z. VOJTOVÁ, L. MICHLOVSKÁ, L.

Original Title

PLGA-PEG-PLGA Core-Shell Nanoparticles for Antibacterial Enzyme Delivery: A Promising Approach for Enhancing Calcium Phosphate Cements

Type

abstract

Language

English

Original Abstract

Fixation of small bone fragments, especially those smaller than 1 cm, remains a challenge in orthopaedic surgery as these pieces are often discarded due to difficulties in stabilization. Calcium phosphate bone cements offer a promising solution for bonding these small fragments back to the bone, due to their biocompatibility and osteoconductivity. However, their mechanical properties and degradation rates can be further optimized by incorporating the triblock copolymer PLGA-PEG-PLGA (poly(lactic-co-glycolic acid)-b-poly(ethylene glycol)-b-poly(lactic-co-glycolic acid)) into the cement formulation. Additionally, to ensure an infection-free healing process, antibacterial enzymes can be added. Encapsulating these enzymes in core-shell nanoparticles enhances their stability and enables gradual release, allowing for targeted antibacterial action at the implantation site. This study aimed to develop core-shell nanoparticles using PLGA-PEG-PLGA copolymer encapsulating antibacterial enzymes, lysozyme and lysostaphin, for potential incorporation into calcium phosphate bone cement. The nanoparticles were prepared using the double emulsion (W/O/W) solvent evaporation method and characterized using scanning transmission electron microscopy (STEM) to confirm their spherical shape. Stability tests and in vitro release studies revealed that lysozymeencapsulated nanoparticles maintained stability over 21 days, conversely, lysostaphin-loaded nanoparticles showed stability for only 3 days, followed by destabilization. Antimicrobial testing demonstrated that lysostaphin-loaded nanoparticles retained significant antibacterial activity. These findings highlight the potential of PLGA-PEG-PLGA core-shell nanoparticles as a promising approach for enhancing the effectiveness of calcium phosphate bone cements in orthopaedic applications. The encapsulation strategy not only improves enzyme stability but also facilitates controlled release, contributing to better infection control and bone healing.

Keywords

core-shell nanoparticles, antibacterial enzymes, thermosensitive copolymer, lysostaphin, lysozyme

Authors

KREUZEROVÁ, M.; TUŠLOVÁ, K.; KADLECOVÁ, Z.; VOJTOVÁ, L.; MICHLOVSKÁ, L.

Released

23. 4. 2025

Location

Praha

ISBN

978-80-907779-4-1

Book

XVI. mezinárodní konference Bioimplantologie a regenerativní medicína

Edition number

1

Pages from

56

Pages to

56

Pages count

80

URL

https://921c88450d.clvaw-cdnwnd.com/17e12706bb26b88046060a8921d16f31/200000714-ad5cbad5cd/Program_a_sbornik_abstraktu_Bioimplantologie_2025.pdf?ph=921c88450d

BibTex

@misc{BUT198356,
  author="Monika {Kreuzerová} and Klára {Tušlová} and Zuzana {Kadlecová} and Lenka {Michlovská} and Lucy {Vojtová}",
  title="PLGA-PEG-PLGA Core-Shell Nanoparticles for Antibacterial Enzyme Delivery: A Promising 
Approach for Enhancing Calcium Phosphate Cements",
  booktitle="XVI. mezinárodní konference Bioimplantologie a regenerativní medicína",
  year="2025",
  edition="1",
  pages="56--56",
  address="Praha",
  isbn="978-80-907779-4-1",
  url="https://921c88450d.clvaw-cdnwnd.com/17e12706bb26b88046060a8921d16f31/200000714-ad5cbad5cd/Program_a_sbornik_abstraktu_Bioimplantologie_2025.pdf?ph=921c88450d",
  note="abstract"
}