Publication detail

Finishing the S.pombe genome deletion project. An update.

ČARNECKÁ, M. MÁROVÁ, I.

Original Title

Finishing the S.pombe genome deletion project. An update.

Type

journal article - other

Language

English

Original Abstract

The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. the collection of deletion mutants now covers 99% of the fission yeast open reading frames.

Keywords

fission yeast; gene deletion; genomics; essential genes; vectors

Authors

ČARNECKÁ, M.; MÁROVÁ, I.

RIV year

2010

Released

28. 7. 2010

Publisher

LANDES BIOSCIENCE

Location

USA

ISBN

1538-4101

Periodical

Cell Cycle

Year of study

9

Number

12

State

United States of America

Pages from

2399

Pages to

2402

Pages count

4

BibTex

@article{BUT50225,
  author="Martina {Čarnecká} and Ivana {Márová}",
  title="Finishing the S.pombe genome deletion project. An update.",
  journal="Cell Cycle",
  year="2010",
  volume="9",
  number="12",
  pages="2399--2402",
  issn="1538-4101"
}