MRC - Laboratories

Laboratoryofbiotechnologyandbiomaterials

The research work of the Laboratory of Biotechnology, Biomaterials and Natural Substances is carried out in several main areas, which partially overlap and may share some methods and procedures. Research is oriented towards the chemistry, analysis and technology of foods and natural substances, the use of biotechnology and nanotechnology in the production of modern foods and raw materials, and the application of molecular biotechnology to the identification and characterisation of selected foods and supplements. Emphasis is placed in particular on a comprehensive approach to problem solving, combining appropriate progressive techniques to assess problems overall and to propose adequate solutions. The development of modern food sciences is dependent on the development and application of advanced methods for the preparation, characterization and processing of foods and raw materials and the analysis of all types of contents, including the assessment of effects on the body. Modern and sophisticated procedures are being developed for use in the production and optimisation of individual steps in the chemistry and technology of natural substances and foods, as well as in the control of the quality, sensory profile and safety of foods and their ingredients, food raw materials and final products. Considerable attention is also paid to the processing of waste raw materials from the agro-food complex and their evaluation in the biotechnological production of industrially important metabolites such as bioethanol, bioplastics, enzymes and other specific metabolites. Currently, interdisciplinary collaboration is intensifying with the overlap of chemical sciences into the life sciences.

prof. RNDr. Ivana Márová, CSc.

prof. RNDr.

IvanaMárová

CSc.

Head of group

+420 54114 9351, 9352
marova@fch.vut.cz

Ing. Jaromír Pořízka, Ph.D.

Ing.

JaromírPořízka

Ph.D.

Contact person

+420541149320
porizka@fch.vut.cz

  • Research scope

    • Biotechnological production of bioplastics on the basis of polyhydroxyalkanoates, enzymes, provitamins, carotenoids and other metabolites including enriched microbial biomass
    • Valorization of food industry waste substrates, agricultural and other productions
    • Purification, extraction and isolation of biomolecules and biopolymers from natural materials, molecular characterization of these molecules by means of advanced instrumental techniques
    • Cultivation of photosynthetic microorganisms – algae and cyanobactreriae for the production of healthy biomass
    • Engaging selected metabolites in the stress response of microorganisms
    • Physiology of microorganisms and its regulation
    • Monitoring and optimization of biotechnological processes, transfer to industrial scale (up-stream)
    • Utilization of advanced techniques to analyse viability of microbial cells
    • Preparation, characterization and application of micro/nano fibres and micro/nano particles from biomaterials and utilization of these products
    • Utilization of microorganisms in biodegradation processes
    • Determining antimicrobial effects of natural substances on tested microorganisms
    • Determining potential toxic effects of natural substances and nanomaterials on human cells (cytotoxicity) and cell systems (DNA – genotoxicity) in order to assess the safety of their use
  • Selected projects

  • Selected publications

    • OBRUČA, S.; DVOŘÁK, P.; SEDLÁČEK, P.; KOLLER, M.; SEDLÁŘ, K.; PERNICOVÁ, I.; ŠAFRÁNEK, D. Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics. BIOTECHNOLOGY ADVANCES, 2022, roč. 58, č. 9, s. 1-28. ISSN: 0734-9750.
    • PERNICOVÁ, I.; KOUŘILOVÁ, X.; NOVÁČKOVÁ, I.; SEDLÁČEK, P.; OBRUČA, S. Production of P(3HB-co-4HB) copolymer by new thermophilic isolate Aneurinibacillus sp. H1 in batch, repated-batch and fed-batch cultivation modes of cultivation. Biotechnology and Biotechnological Equipment. 2021. s. 1 (1 s.). ISSN: 1314-3530.
    • DZURENDOVÁ, S.; SHAPAVAL, V.; TAFINTSEVA, V.; KOHLER, A.; BYRTUSOVÁ, D.; SZOTKOWSKI, M.; MÁROVÁ, I.; ZIMMERMANN, B. Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy. Microorganisms, 2021, roč. 22, č. 13, s. 1-23. ISSN: 2076-2607.
    • SZOTKOWSKI, M.; HOLUB, J.; ŠIMANSKÝ, S.; HUBAČOVÁ, K.; SIKOROVÁ, P.; MARINIČOVÁ, V.; NĚMCOVÁ, A.; MÁROVÁ, I. Bioreactor Co-Cultivation of High Lipid and Carotenoid Producing Yeast Rhodotorula kratochvilovae and Several Microalgae under Stress. Microorganisms, 2021, roč. 9, č. 6, s. 1-23. ISSN: 2076-2607.
    • BYRTUSOVÁ, D.; SZOTKOWSKI, M.; KUROWSKA, K.; SHAPAVAL, V.; MÁROVÁ, I. Rhodotorula kratochvilovae CCY 20-2-26-The Source of Multifunctional Metabolites. Microorganisms, 2021, roč. 9, č. 6, s. 1-18. ISSN: 2076-2607.
    • SLANINOVÁ, E.; RUBANOVÁ, B.; ČERNAYOVÁ, D.; NOVÁČKOVÁ, I.; HAVLÍKOVÁ, M.; MRÁZOVÁ, K.; SEDLÁČEK, P.; OBRUČA, S. Study of aeration and light/dark conditions on production of polyhydroxyalkanoates in bacteria Rhodospirillium rubrum. 2022. s. 1 (1 s.).
    • SLANINOVÁ, E.; ČERNAYOVÁ, D.; ŠEDRLOVÁ, Z.; MRÁZOVÁ, K.; SEDLÁČEK, P.; NEBESÁŘOVÁ, J.; OBRUČA, S. Instrumental characterization of Cyanobacteria as Polyhydroxybutyrate Producer. World Academy of Science, Engineering and Technology. 2021. s. 75-75. ISSN: 1307-6892.
    • ČERNAYOVÁ, D.; SÚKENÍK, M.; SLANINOVÁ, E.; SEDLÁČEK, P.; OBRUČA, S. Generation of New Bio-Inoculants Using Self-encapsulation of Plant-growth Promoting Bacteria Azotobacter vinelandii. The Biomania Student Scientific Meeting. 1st. Brno, Czech Republic; 1st edition 2022: Masaryk University Press, 2022. s. 112-112. ISBN: 978-80-280-0040-0.
    • OBRUČA, S.; DVOŘÁK, P.; SEDLÁČEK, P.; KOLLER, M.; SEDLÁŘ, K.; PERNICOVÁ, I.; ŠAFRÁNEK, D. Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics. BIOTECHNOLOGY ADVANCES, 2022, roč. 58, č. 9, s. 1-28. ISSN: 0734-9750.
    • KOVALČÍK, A.; PERNICOVÁ, I.; OBRUČA, S.; SZOTKOWSKI, M.; ENEV, V.; KALINA, M.; MÁROVÁ, I. Grape winery waste as a promising feedstock for the production of polyhydroxyalkanoates and other value-added products. FOOD AND BIOPRODUCTS PROCESSING, 2020, roč. 124, č. 1, s. 1-10. ISSN: 0960-3085.
    • Other publications
  • Patents and utility models

    • MÁROVÁ, I.; OBRUČA, S.; PŘIKRYL, R.; VUT v Brně: Způsob produkce polyhydroxyalkanoátů (PHA) na olejovém substrátu. 304183, patent. (2013)
    • OBRUČA, S.; PERNICOVÁ, I.; KUČERA, D.; NOVÁČKOVÁ, I.; SEDLÁČEK, P.; Vysoké učení technické v Brně, Brno, CZ: Způsob výroby polyhydroxyalkanoátů pomocí isolátu termofilního bakteriálního kmene Aneurinibacillus sp. H1. 308626, patent. (2021)
    • MÁROVÁ, I.; KUNDRÁT, V.; VUT v Brně: Způsob rozpouštění biodegradabilních polyesterů. 308735, patent. (2021)
    • MÁROVÁ, I.; POSPÍŠIL, L.; KUNDRÁT, V.; VUT v Brně: Polymerní vlákno a způsob jeho přípravy. 306448, patent. (2016)
    • HÁRONIKOVÁ, A.; MÁROVÁ, I.; KOSTOVOVÁ, I.; Vysoké učení technické v Brně: Suchá biomasa karotenogenních kvasinek druhu C.capitatum CCY10-1-2 pro krmivářské účely. 28679, užitný vzor. (2015)
  • Services offered

    • Determining levels of biologically active substances in various matrixes
    • Determining activity of enzyme and microbiological specimens
    • Determining biological effects
    • Determining antimicrobial activity
    • Determining genotoxicity and cytotoxicity of encapsulation of biologically active substances
    • Optimization of the individual steps of biotechnological processes (up-stream processes, cultivations, down-stream processes)
    • Designing and developing possible strategies for exploitation of waste and by-products of food and other production
    • Preparation of materials on the basis of nano/micro fibres and nano/micro particles for a broad spectrum of applications.

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