Membranesystems
The research group focuses on the preparation and study of membrane systems based on phospholipids, ionic amphiphilic pairs and their complexes with other biocolloids, especially biopolymers, intended for use as carriers of biologically active substances. Membrane systems include vesicles/liposomes and supported lipid bilayers. An integral part of the research is also the development of stationary and time-resolved fluorescence spectroscopy and time-resolved fluorescence microscopy for the study of the above-mentioned systems.
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Research team
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Research focus
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Phospholipid-based membrane systems
We focus on the preparation and characterisation of liposomes, vesicles and supported lipid bilayers intended for the transport of biologically active substances. -
Carriers of biologically active substances
We develop membrane carrier systems based on phospholipids, ionic amphiphilic pairs and biopolymers, with an emphasis on stability and efficient drug delivery. -
Inhalation forms of carrier systems
In cooperation with the Faculty of Mechanical Engineering at Brno University of Technology, we prepare inhalation formulations of membrane systems in the form of solutions and solid particles for the transport of active substances into the lungs. -
Formulations without chlorinated solvents
We currently focus on the development of membrane systems prepared without the use of chlorinated solvents, with regard to safety and sustainability. -
Fluorescence spectroscopy and microscopy
We develop the use of steady-state and time-resolved fluorescence spectroscopy and fluorescence microscopy for the study of the structure, stability and interactions of membrane systems. -
Stimuli-responsive membrane complexes
The aim of the research is to design carriers responsive to physicochemical stimuli, enabling targeted release of biologically active substances according to the intended application.
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Phospholipid-based membrane systems
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Selected publications
- Patent: Method for preparing a physically crosslinked hydrogel with at least one solubilised hydrophobic compound. VaV ID: 169361
- N,N,N-Trimethyl chitosan as a permeation enhancer for inhalation drug delivery: Interaction with a model pulmonary surfactant. VaV ID: 183228
- Liposomal Form of Erlotinib for Local Inhalation Administration and Efficiency of Its Transport to the Lungs. VaV ID: 182463
- Nebulization and In Vitro Upper Airway Deposition of Liposomal Carrier Systems. VaV ID: 188404
- Long circulating liposomal platform utilizing hydrophilic polymer-based surface modification: preparation, characterisation, and biological evaluation.
- The immunogenicity of p24 protein from HIV-1 virus is strongly supported and modulated by coupling with liposomes and mannan.
- KADLEC, M.; PEKAŘ, M.; SMILEK, J. Mechanical Properties of Agarose Hydrogels Tuned by Amphiphilic Structures. Colloids and Surfaces. A, Physicochemical and Engineering Aspects, 2024, vol. 700, no. 1, 14 p. ISSN: 0927-7757. VaV ID: 189041
- More publications
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Selected projects
- GC19-14024J – Hyaluronan-catanionic vesicles complexes – interaction, structure, physical property, and stability
- GF19-29651L – The biological role of poly-hydroxyalkanoates in cyanobacteria
- More projects
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Offered services and analyses
- Formulation of carrier systems for water-insoluble biologically active substances.
- Study of physicochemical properties and stability of carrier systems.
- Determination of encapsulation efficiency of biologically active substances in carrier systems.
- Measurement of fluorescence characteristics of materials, including emission and excitation spectra, determination of fluorescence quantum yield, determination of fluorescence and phosphorescence lifetime (ps–s range), imaging of cellular structures using FLIM and anisotropic imaging techniques, study of diffusion properties of fluorescent materials using FCS, and more.
