Publication result detail
Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns
ŠESTÁK, J.; KAHLE, V.
Original Title
Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns
English Title
Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns
Type
WoS Article
Original Abstract
In this short communication, a low cost and easily feasible pressure-controlled extension of the previously described simple gradient liquid chromatography platform is proposed. A test mixture of four nitro esters was separated by 10-60%v/v acetone/water gradient and a high repeatability of retention volumes at 20 MPa (RSD less than 0.45%) was realized. Separations were also performed at different values of pressure (20, 25, and 31 MPa), and only small variations of the retention volumes (up to 0.8%) were observed. In this particular case, the gain in the analysis speed of 7% compared to the constant flow mode was realized at a constant pressure.
English abstract
In this short communication, a low cost and easily feasible pressure-controlled extension of the previously described simple gradient liquid chromatography platform is proposed. A test mixture of four nitro esters was separated by 10-60%v/v acetone/water gradient and a high repeatability of retention volumes at 20 MPa (RSD less than 0.45%) was realized. Separations were also performed at different values of pressure (20, 25, and 31 MPa), and only small variations of the retention volumes (up to 0.8%) were observed. In this particular case, the gain in the analysis speed of 7% compared to the constant flow mode was realized at a constant pressure.
Keywords
Constant pressure HPLC; Gradient elution; Simple liquid chromatograph; Nano columns; Nitro esters
Key words in English
Constant pressure HPLC; Gradient elution; Simple liquid chromatograph; Nano columns; Nitro esters
Authors
ŠESTÁK, J.; KAHLE, V.
RIV year
2015
Released
11.06.2014
ISBN
0021-9673
Periodical
JOURNAL OF CHROMATOGRAPHY A
Volume
1350
Number
6
State
Kingdom of the Netherlands
Pages from
68
Pages to
71
Pages count
4
BibTex
@article{BUT107541,
author="Jozef {Šesták} and Vladislav {Kahle}",
title="Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns",
journal="JOURNAL OF CHROMATOGRAPHY A",
year="2014",
volume="1350",
number="6",
pages="68--71",
issn="0021-9673"
}