R&D result detail

Original Title

Determination of seventeen mycotoxins in barley and malt in the Czech Republic

English Title

Determination of seventeen mycotoxins in barley and malt in the Czech Republic

Type

WoS Article

Original Abstract

In this study, an analytical method for the determination of 17 mycotoxins was developed and validated: common (aflatoxins B1, B2, G1, and G2, fumonisins B1 and B2, ochratoxin A, deoxynivalenol, nivalenol, zearalenon, T-2 and HT-2 toxin) and "emerging" toxins (enniatins A, A1, B, and B1, and beauvericin) were detected using ultra-performance liquid chromatography with mass spectrometry. A modified QuEChERS method was used for extraction. The method was applied on a total set of 52 samples of barley and malt. 100 % of samples were contaminated with at least one of mycotoxins. None of the investigated samples contained any of four aflatoxins nor ochratoxin A. Fumonisin B1 occurred only in one sample, Fumonisin B2 and zearalenon were found in two barley samples. Enniatins were detected in all samples. No sample exceeded the maximum allowable limit (MAL) set for selected mycotoxins in unprocessed or processed cereals by the European Union.

English abstract

In this study, an analytical method for the determination of 17 mycotoxins was developed and validated: common (aflatoxins B1, B2, G1, and G2, fumonisins B1 and B2, ochratoxin A, deoxynivalenol, nivalenol, zearalenon, T-2 and HT-2 toxin) and "emerging" toxins (enniatins A, A1, B, and B1, and beauvericin) were detected using ultra-performance liquid chromatography with mass spectrometry. A modified QuEChERS method was used for extraction. The method was applied on a total set of 52 samples of barley and malt. 100 % of samples were contaminated with at least one of mycotoxins. None of the investigated samples contained any of four aflatoxins nor ochratoxin A. Fumonisin B1 occurred only in one sample, Fumonisin B2 and zearalenon were found in two barley samples. Enniatins were detected in all samples. No sample exceeded the maximum allowable limit (MAL) set for selected mycotoxins in unprocessed or processed cereals by the European Union.

Keywords

mycotoxins, QuEChERS, mass spectrometry, malting barley, ELISA

Key words in English

mycotoxins, QuEChERS, mass spectrometry, malting barley, ELISA

Authors

BOLECHOVÁ, M.; BENEŠOVÁ, K.; BĚLÁKOVÁ, S.; ČÁSLAVSKÝ, J.; POSPÍCHALOVÍ, M.; MIKULÍKOVÁ, R.

RIV year

2016

Released

01.01.2015

Publisher

Elsevier

ISBN

0956-7135

Periodical

FOOD CONTROL

Volume

47

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

108

Pages to

113

Pages count

6

BibTex

@article{BUT108917,
  author="BOLECHOVÁ, M. and BENEŠOVÁ, K. and BĚLÁKOVÁ, S. and ČÁSLAVSKÝ, J. and POSPÍCHALOVÍ, M. and MIKULÍKOVÁ, R.",
  title="Determination of seventeen mycotoxins in barley and malt in the Czech Republic",
  journal="FOOD CONTROL",
  year="2015",
  volume="47",
  number="1",
  pages="108--113",
  doi="10.1016/j.foodcont.2014.06.045",
  issn="0956-7135"
}