Publication result detail
The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes
MICHALICOVÁ, P.; BORŮVKOVÁ, T.; MRAVEC, F.; ČÁSLAVSKÝ, J.; PEKAŘ, M.
Original Title
The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes
English Title
The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes
Type
Abstract
Original Abstract
Hyaluronan is a native biopolymer with hydrophilic character, which contains on its chain also hydrophobic areas. Due to the prevalent hydrophilicity these parts are protected by organized water molecules. For accessing the hydrophobic parts for interactions with hydrophobic fluorescence probes the method of drying was used. During the preparation of samples for drying tert-butanol was added. The problem arise if residual part of tert-butanol remains in the sample due to its hydrophobic organic character, which can significantly affect the result of fluorescence measurement. The amount of residual tert-butanol was determined by head-space extraction with gas chromatography detection.
English abstract
Hyaluronan is a native biopolymer with hydrophilic character, which contains on its chain also hydrophobic areas. Due to the prevalent hydrophilicity these parts are protected by organized water molecules. For accessing the hydrophobic parts for interactions with hydrophobic fluorescence probes the method of drying was used. During the preparation of samples for drying tert-butanol was added. The problem arise if residual part of tert-butanol remains in the sample due to its hydrophobic organic character, which can significantly affect the result of fluorescence measurement. The amount of residual tert-butanol was determined by head-space extraction with gas chromatography detection.
Keywords
hyaluronan, drying, tert-butanol, gas chromatography
Key words in English
hyaluronan, drying, tert-butanol, gas chromatography
Authors
MICHALICOVÁ, P.; BORŮVKOVÁ, T.; MRAVEC, F.; ČÁSLAVSKÝ, J.; PEKAŘ, M.
Released
07.09.2014
Publisher
Czech Chemical Society
Book
Chemické listy
ISBN
0009-2770
Periodical
CHEMICKE LISTY
Volume
108
Number
8
State
Czech Republic
Pages from
772
Pages to
772
Pages count
1
BibTex
@misc{BUT109297,
author="Petra {Michalicová} and Tereza {Borůvková} and Filip {Mravec} and Josef {Čáslavský} and Miloslav {Pekař}",
title="The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes",
booktitle="Chemické listy",
year="2014",
journal="CHEMICKE LISTY",
volume="108",
number="8",
pages="772--772",
publisher="Czech Chemical Society",
issn="0009-2770",
note="Abstract"
}