Publication result detail
Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types
KALBÁČOVÁ, M.; VERDÁNOVÁ, M.; MRAVEC, F.; HALASOVÁ, T.; PEKAŘ, M.
Original Title
Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types
English Title
Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types
Type
WoS Article
Original Abstract
The effect of different concentrations of CTAB (in the range of 0.2 mM – 2 mM, i.e. including its critical micelle concentration) on viability of selected human cells (osteoblasts and keratinocytes) was studied by a variety of methods (immunocytochemical and biochemical), testing the cell viability and the metabolism, to get a complex overview. All of the used methods confirmed the cytotoxic effect of CTAB, which could, however, be suppressed by the presence of hyaluronan (molecular weight 806 kDa, in the concentration of 1 g/l) in the case of the lowest CTAB concentration used (0.2 mM) when the fetal bovine serum was also present in the cultivation medium. Thus it could be concluded that hyaluronan can be used as successful protector of specific cell types against cytotoxic CTAB at low concentrations.
English abstract
The effect of different concentrations of CTAB (in the range of 0.2 mM – 2 mM, i.e. including its critical micelle concentration) on viability of selected human cells (osteoblasts and keratinocytes) was studied by a variety of methods (immunocytochemical and biochemical), testing the cell viability and the metabolism, to get a complex overview. All of the used methods confirmed the cytotoxic effect of CTAB, which could, however, be suppressed by the presence of hyaluronan (molecular weight 806 kDa, in the concentration of 1 g/l) in the case of the lowest CTAB concentration used (0.2 mM) when the fetal bovine serum was also present in the cultivation medium. Thus it could be concluded that hyaluronan can be used as successful protector of specific cell types against cytotoxic CTAB at low concentrations.
Keywords
Cetyltrimethylammonium bromide; Cytoprotection; Cytotoxicity; Hyaluronan; Keratinocyte; Osteoblast
Key words in English
Cetyltrimethylammonium bromide; Cytoprotection; Cytotoxicity; Hyaluronan; Keratinocyte; Osteoblast
Authors
KALBÁČOVÁ, M.; VERDÁNOVÁ, M.; MRAVEC, F.; HALASOVÁ, T.; PEKAŘ, M.
RIV year
2015
Released
08.09.2014
ISBN
0927-7757
Periodical
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
Volume
460
Number
1
State
Kingdom of the Netherlands
Pages from
204
Pages to
208
Pages count
5
BibTex
@article{BUT109678,
author="KALBÁČOVÁ, M. and VERDÁNOVÁ, M. and MRAVEC, F. and HALASOVÁ, T. and PEKAŘ, M.",
title="Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types",
journal="COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS",
year="2014",
volume="460",
number="1",
pages="204--208",
issn="0927-7757"
}