Publication result detail

Interaction of E6 Gene from Human Papilloma Virus 16 (HPV-16) with CdS Quantum Dots

NEJDL, L.; SKALIČKOVÁ, S.; KUDR, J.; RUTTKAY-NEDECKÝ, B.; NGUYEN, H.; MERLOS RODRIGO, M.; KOPEL, P.; NOVOTNÁ, M.; ADAM, V.; KIZEK, R.

Original Title

Interaction of E6 Gene from Human Papilloma Virus 16 (HPV-16) with CdS Quantum Dots

English Title

Interaction of E6 Gene from Human Papilloma Virus 16 (HPV-16) with CdS Quantum Dots

Type

WoS Article

Original Abstract

The aim of this study was to analyze the interactions of blue and yellow fluorescent CdS quantum dots (CdS-QDs) with human papillomavirus 16 (HPV-16) oncogene E6. The interactions were investigated using chip capillary electrophoresis, spectrophotometry and square wave voltammetry (SWV). Using chip capillary electrophoresis we proved that blue fluorescent CdS-QDs (0.5 mM) caused an increase of the migration time of the E6 HPV-16 DNA–CdS-QDs complex by 42 s compared to control DNA (E6 HPV-16). The same concentration of yellow fluorescent CdS-QDs caused an increase in the migration time of the DNA–CdS-QDs complex by 108 s compared to the control DNA (E6 HPV-16). The difference in the migration times between both complexes was 66 s. Using square wave voltammetry (SWV), the reduction signal of cytosine and adenine (peak CA) was observed.

English abstract

The aim of this study was to analyze the interactions of blue and yellow fluorescent CdS quantum dots (CdS-QDs) with human papillomavirus 16 (HPV-16) oncogene E6. The interactions were investigated using chip capillary electrophoresis, spectrophotometry and square wave voltammetry (SWV). Using chip capillary electrophoresis we proved that blue fluorescent CdS-QDs (0.5 mM) caused an increase of the migration time of the E6 HPV-16 DNA–CdS-QDs complex by 42 s compared to control DNA (E6 HPV-16). The same concentration of yellow fluorescent CdS-QDs caused an increase in the migration time of the DNA–CdS-QDs complex by 108 s compared to the control DNA (E6 HPV-16). The difference in the migration times between both complexes was 66 s. Using square wave voltammetry (SWV), the reduction signal of cytosine and adenine (peak CA) was observed.

Keywords

Chip capillary electrophoresis, Square wave, voltammetry DNA interaction, human papilloma virus, E6 HPV-16 gene, Cadmium sulphide quantum dots

Key words in English

Chip capillary electrophoresis, Square wave, voltammetry DNA interaction, human papilloma virus, E6 HPV-16 gene, Cadmium sulphide quantum dots

Authors

NEJDL, L.; SKALIČKOVÁ, S.; KUDR, J.; RUTTKAY-NEDECKÝ, B.; NGUYEN, H.; MERLOS RODRIGO, M.; KOPEL, P.; NOVOTNÁ, M.; ADAM, V.; KIZEK, R.

Released

01.11.2014

ISBN

0009-5893

Periodical

CHROMATOGRAPHIA

Volume

77

Number

21-22

State

Federal Republic of Germany

Pages from

1433

Pages to

1439

Pages count

7

Full text in the Digital Library

http://hdl.handle.net/

BibTex

@article{BUT110911,
  author="Lukáš {Nejdl} and Sylvie {Skaličková} and Jiří {Kudr} and Branislav {Ruttkay-Nedecký} and Hoai Viet {Nguyen} and Miguel Ángel {Merlos Rodrigo} and Pavel {Kopel} and Marie {Novotná} and Vojtěch {Adam} and René {Kizek}",
  title="Interaction of E6 Gene from Human Papilloma Virus 16 (HPV-16) with CdS Quantum Dots",
  journal="CHROMATOGRAPHIA",
  year="2014",
  volume="77",
  number="21-22",
  pages="1433--1439",
  doi="10.1007/s10337-014-2734-5",
  issn="0009-5893"
}