Publication result detail
Optimisation of DGGE nested PCR for red yeasts characterisation
KOSTOVOVÁ, I.; HÁRONIKOVÁ, A.; PETRIK, S.; MÁROVÁ, I.
Original Title
Optimisation of DGGE nested PCR for red yeasts characterisation
English Title
Optimisation of DGGE nested PCR for red yeasts characterisation
Type
Abstract
Original Abstract
Carotenogenic yeasts are a diverse group of unrelated organisms (mostly Basidiomycota) that can be found in soil, fresh and marine water, on plants, commonly associated with animals and man-made habitant. In this work the PCR - denaturing gradient gel electrophoresis (DGGE) method was optimized for characterisation of red yeast strains. For this usage, inter-specific variables of strongly conserved sequences of genomic DNA, especially ITS1 and 5.8-ITS2 rDNA regions were amplified. At all, nine of carotenogenic yeast strains of the genus Rhodotorula, Sporobolomyces, Cystofilobasidium and Phaffia were analyzed.
English abstract
Carotenogenic yeasts are a diverse group of unrelated organisms (mostly Basidiomycota) that can be found in soil, fresh and marine water, on plants, commonly associated with animals and man-made habitant. In this work the PCR - denaturing gradient gel electrophoresis (DGGE) method was optimized for characterisation of red yeast strains. For this usage, inter-specific variables of strongly conserved sequences of genomic DNA, especially ITS1 and 5.8-ITS2 rDNA regions were amplified. At all, nine of carotenogenic yeast strains of the genus Rhodotorula, Sporobolomyces, Cystofilobasidium and Phaffia were analyzed.
Keywords
DGGE; PCR; red yeast
Key words in English
DGGE; PCR; red yeast
Authors
KOSTOVOVÁ, I.; HÁRONIKOVÁ, A.; PETRIK, S.; MÁROVÁ, I.
Released
20.05.2014
Book
41st annual conference on yeast
Pages from
55
Pages to
55
Pages count
86
BibTex
@misc{BUT111756,
author="Iveta {Kostovová} and Andrea {Němcová} and Siniša {Petrik} and Ivana {Márová}",
title="Optimisation of DGGE nested PCR for red yeasts characterisation",
booktitle="41st annual conference on yeast",
year="2014",
pages="55--55",
note="Abstract"
}