Publication result detail
Determination of the reference genes in Streptococcus equi subsp. zooepidemicus for real-time PCR
HRUDÍKOVÁ, R. PEPELIAEV, S.
Original Title
Determination of the reference genes in Streptococcus equi subsp. zooepidemicus for real-time PCR
English Title
Determination of the reference genes in Streptococcus equi subsp. zooepidemicus for real-time PCR
Type
Abstract
Original Abstract
The whole genome expression analysis of Streptococcus equi subsp. zooepidemicus strain ATCC 35246 was performed using DNA microarrays. According to the stability of gene expression 10 genes including gyrA and gyrB were chosen as potential reference genes. Further analysis of expression stability of selected genes was done by real-time PCR. Ct values from PCR analysis were processed in Microsoft Excel and our conclusions were additionally confirmed by GeNorm normalization. Reference gene was chosen
English abstract
The whole genome expression analysis of Streptococcus equi subsp. zooepidemicus strain ATCC 35246 was performed using DNA microarrays. According to the stability of gene expression 10 genes including gyrA and gyrB were chosen as potential reference genes. Further analysis of expression stability of selected genes was done by real-time PCR. Ct values from PCR analysis were processed in Microsoft Excel and our conclusions were additionally confirmed by GeNorm normalization. Reference gene was chosen
Keywords
Reference genes, real-time PCR, whole genome expression
Key words in English
Reference genes, real-time PCR, whole genome expression
Authors
HRUDÍKOVÁ, R. PEPELIAEV, S.
RIV year
2016
Released
23.04.2015
Publisher
Masaryk University
Location
Brno
ISBN
978-80-210-7825-3
Book
CEITEC PhD Retreat, Book of Abstracts
Pages from
49
Pages to
49
Pages count
1
BibTex
@misc{BUT115656,
author="Radka {Hrudíková}",
title="Determination of the reference genes in Streptococcus equi subsp. zooepidemicus for real-time PCR",
booktitle="CEITEC PhD Retreat, Book of Abstracts",
year="2015",
pages="49--49",
publisher="Masaryk University",
address="Brno",
isbn="978-80-210-7825-3",
note="Abstract"
}