Result with impact on practice detail
Comparison of magnetic nano- and microparticles application for DNA isolation from real samples
KONEČNÁ, J.; ŠPANOVÁ, A.; HORÁK, D.; RITTICH, B.
Original Title
Comparison of magnetic nano- and microparticles application for DNA isolation from real samples
English Title
Comparison of magnetic nano- and microparticles application for DNA isolation from real samples
Type
Audiovisual work
Original Abstract
In the past decade, magnetic nanoparticles and mickroparticles have been widely employed for numerous biomedical and biological applications. The aim of this work was to compare the application of poly (L-lysine) (PLL) modified iron oxide nanoparticles and hydrophilic magnetic non-porus poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate- P(HEMA-co-GMA) microsphere for DNA isolation from food supplements. Conventional phenol extraction procedure was used as control. Phosphate buffer (pH 7,8) and TE buffer containing 10mM KCl (pH 9.0) and phosphate buffer (pH 8.0) were used fot DNA adsorption /elution in the case of poly (L-lysine) (PLL) modified iron oxide nanoparticles; mixture 16% PEG 6,000 and 2.0 M NaCl and TE buffer were used for DNA adsorption/elution in case of P(HEMA-co-GMA) microsphere. Method was applied for DNA isolation from following food supplements: Linex®forte, Laktobacily forte, Pangamin and Lactomax. Quantity of extracted DNA was checked spectrofotometrically and by PCR. DNAeEluted from both tested magnetic particles was in PCR – ready quality.
English abstract
In the past decade, magnetic nanoparticles and mickroparticles have been widely employed for numerous biomedical and biological applications. The aim of this work was to compare the application of poly (L-lysine) (PLL) modified iron oxide nanoparticles and hydrophilic magnetic non-porus poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate- P(HEMA-co-GMA) microsphere for DNA isolation from food supplements. Conventional phenol extraction procedure was used as control. Phosphate buffer (pH 7,8) and TE buffer containing 10mM KCl (pH 9.0) and phosphate buffer (pH 8.0) were used fot DNA adsorption /elution in the case of poly (L-lysine) (PLL) modified iron oxide nanoparticles; mixture 16% PEG 6,000 and 2.0 M NaCl and TE buffer were used for DNA adsorption/elution in case of P(HEMA-co-GMA) microsphere. Method was applied for DNA isolation from following food supplements: Linex®forte, Laktobacily forte, Pangamin and Lactomax. Quantity of extracted DNA was checked spectrofotometrically and by PCR. DNAeEluted from both tested magnetic particles was in PCR – ready quality.
Keywords
DNA isolation, magnetic poly (L-lysine) modified iron oxide nanoparticles, magnetic P(HEMA-co-GMA) microsphere, PCR
Key words in English
DNA isolation, magnetic poly (L-lysine) modified iron oxide nanoparticles, magnetic P(HEMA-co-GMA) microsphere, PCR
Authors
KONEČNÁ, J.; ŠPANOVÁ, A.; HORÁK, D.; RITTICH, B.
RIV year
2016
Released
03.12.2015
ISBN
978-80-214-5290-6
BibTex
@misc{BUT120985,
author="Jana {Konečná} and Alena {Španová} and Daniel {Horák} and Bohuslav {Rittich}",
title="Comparison of magnetic nano- and microparticles application for DNA isolation from real samples",
year="2015",
isbn="978-80-214-5290-6",
note="Audiovisual work"
}