Publication result detail
Determination of the Reference Genes in Streptococcus equi subsp. zooepidemicus for Real-Time PCR
Radka Hrudíková, Stanislav Pepeliaev, Vladimír Velebný
Original Title
Determination of the Reference Genes in Streptococcus equi subsp. zooepidemicus for Real-Time PCR
English Title
Determination of the Reference Genes in Streptococcus equi subsp. zooepidemicus for Real-Time PCR
Type
Abstract
Original Abstract
Real-time polymerase chain reaction (PCR) has become the preferred method for studying expression. The study of gene expression profiles of whole genome is commonly performed by relying on such technique like cDNA microarray. Real-time PCR technique is commonly used to study expression of tens of genes. Real-time PCR is considered to be the most accurate, because of its high sensitivity and specificity. However to validate obtained data reference gene is needed. We have studied gene expression of bacterial strains of Streptococcus equi subsp. zooepidemicus (SEZ).
English abstract
Real-time polymerase chain reaction (PCR) has become the preferred method for studying expression. The study of gene expression profiles of whole genome is commonly performed by relying on such technique like cDNA microarray. Real-time PCR technique is commonly used to study expression of tens of genes. Real-time PCR is considered to be the most accurate, because of its high sensitivity and specificity. However to validate obtained data reference gene is needed. We have studied gene expression of bacterial strains of Streptococcus equi subsp. zooepidemicus (SEZ).
Keywords
Real-Time PCR, reference gene, gene expression stability
Key words in English
Real-Time PCR, reference gene, gene expression stability
Authors
Radka Hrudíková, Stanislav Pepeliaev, Vladimír Velebný
RIV year
2016
Released
03.12.2015
Publisher
Vysoké Učení Technické v Brně, Fakulta chemická, Purkyňova 464/118, Brno 61200
Location
Brno
ISBN
978-80-214-5290-9
Book
Sborník abstraktů
Edition
1
Pages from
65
Pages to
65
Pages count
1
BibTex
@misc{BUT121168,
author="Radka {Hrudíková}",
title="Determination of the Reference Genes in Streptococcus equi subsp. zooepidemicus for Real-Time PCR",
booktitle="Sborník abstraktů",
year="2015",
series="1",
edition="1",
pages="65--65",
publisher="Vysoké Učení Technické v Brně, Fakulta chemická, Purkyňova 464/118, Brno 61200",
address="Brno",
isbn="978-80-214-5290-9",
note="Abstract"
}