Publication result detail
Effect of Hyaluronan on Diffusion Properties of Artificial Cell Membrane Studied by Fluorescence Correlation Spectroscopy
ADAMCOVÁ, Z.; MRAVEC, F.; PEKAŘ, M.
Original Title
Effect of Hyaluronan on Diffusion Properties of Artificial Cell Membrane Studied by Fluorescence Correlation Spectroscopy
English Title
Effect of Hyaluronan on Diffusion Properties of Artificial Cell Membrane Studied by Fluorescence Correlation Spectroscopy
Type
Paper in proceedings (conference paper)
Original Abstract
Supported lipid bilayers were used as simplified models of cell membranes to study the effect of hyaluronan solution on their diffusion properties. The bilayers were prepared from lecithin liposomes in phosphate buffer using the vesicle fusion method. Hydrophilized cover glass slides were chosen as the supporting substrate. Two fluorescence probes – Nile Red and DiO were utilized to quantify the diffusion properties of the bilayer with and without presence of hyaluronan. For this purpose Z-scan Fluorescence Correlation Spectroscopy, which is a method developed specially for planar samples, was used. It was found out, that presence of this biopolymer causes slowing of the fluorescence probe diffusion within the bilayer.
English abstract
Supported lipid bilayers were used as simplified models of cell membranes to study the effect of hyaluronan solution on their diffusion properties. The bilayers were prepared from lecithin liposomes in phosphate buffer using the vesicle fusion method. Hydrophilized cover glass slides were chosen as the supporting substrate. Two fluorescence probes – Nile Red and DiO were utilized to quantify the diffusion properties of the bilayer with and without presence of hyaluronan. For this purpose Z-scan Fluorescence Correlation Spectroscopy, which is a method developed specially for planar samples, was used. It was found out, that presence of this biopolymer causes slowing of the fluorescence probe diffusion within the bilayer.
Keywords
Supported lipid bilayers, diffusion, Fluorescence Correlation Spectroscopy, Nile Red, DiO, Hyaluronan
Key words in English
Supported lipid bilayers, diffusion, Fluorescence Correlation Spectroscopy, Nile Red, DiO, Hyaluronan
Authors
ADAMCOVÁ, Z.; MRAVEC, F.; PEKAŘ, M.
RIV year
2017
Released
06.06.2016
Publisher
Masaryk University, Brno
Location
Brno
ISBN
978-80-210-8267-0
Book
XVI. Workshop of Physical Chemists and Electrochemists
Pages from
135
Pages to
138
Pages count
4
BibTex
@inproceedings{BUT128980,
author="Zuzana {Adamcová} and Filip {Mravec} and Miloslav {Pekař}",
title="Effect of Hyaluronan on Diffusion Properties of Artificial Cell Membrane Studied by Fluorescence Correlation Spectroscopy",
booktitle="XVI. Workshop of Physical Chemists and Electrochemists",
year="2016",
pages="135--138",
publisher="Masaryk University, Brno",
address="Brno",
isbn="978-80-210-8267-0"
}