Publication result detail
Production and characterization of extracellular polysaccharide of extremely halophilic Archaea Haloferax mediterranei
CHYTILOVÁ, A.; DRÁBKOVÁ, K.; STREČANSKÁ, P.; ENEV, V.; KUČERA, D.; BENEŠOVÁ, P.; OBRUČA, S.
Original Title
Production and characterization of extracellular polysaccharide of extremely halophilic Archaea Haloferax mediterranei
English Title
Production and characterization of extracellular polysaccharide of extremely halophilic Archaea Haloferax mediterranei
Type
Abstract
Original Abstract
Haloferax mediterranei is extremely halophilic archeon capable of production of two very interesting biopolymers. This microorganism intracellularly accumulates polyhydroxyalkanoates (PHA) while simultaneously excreting polysaccharide. In this work we focused on production and characterization of its extracellular polysaccharide. To decrease cost of the cultivation media we substituted expensive yeast extract by alkaline hydrolyzed waste chicken feather which positively influenced polysaccharide yields.Moreover, isolated polysaccharide was capable to provide very high protective activity for model prokaryotic (Cupriavidus necator) and eukaryotic (Saccharomyces cerevisiae) microorganisms against various stress factors such as freezing, ethanol, high temperature, osmotic pressure, oxidative pressure induced by H2O2 or SDS which imply its interesting biological properties and potential applications in biotechnology or health care.
English abstract
Haloferax mediterranei is extremely halophilic archeon capable of production of two very interesting biopolymers. This microorganism intracellularly accumulates polyhydroxyalkanoates (PHA) while simultaneously excreting polysaccharide. In this work we focused on production and characterization of its extracellular polysaccharide. To decrease cost of the cultivation media we substituted expensive yeast extract by alkaline hydrolyzed waste chicken feather which positively influenced polysaccharide yields.Moreover, isolated polysaccharide was capable to provide very high protective activity for model prokaryotic (Cupriavidus necator) and eukaryotic (Saccharomyces cerevisiae) microorganisms against various stress factors such as freezing, ethanol, high temperature, osmotic pressure, oxidative pressure induced by H2O2 or SDS which imply its interesting biological properties and potential applications in biotechnology or health care.
Keywords
Haloferax mediterranei, Cupriavidus necator, extracellular polysaccharide, Raman spectroscopy, FTIR and elemental analysis
Key words in English
Haloferax mediterranei, Cupriavidus necator, extracellular polysaccharide, Raman spectroscopy, FTIR and elemental analysis
Authors
CHYTILOVÁ, A.; DRÁBKOVÁ, K.; STREČANSKÁ, P.; ENEV, V.; KUČERA, D.; BENEŠOVÁ, P.; OBRUČA, S.
RIV year
2018
Released
11.09.2017
Location
Krakow
Book
6th Central European Congress of Life Sciences Eurobiotech
Pages from
156
Pages to
157
Pages count
1
BibTex
@misc{BUT142931,
author="Aneta {Chytilová} and Kateřina {Drábková} and Paulína {Strečanská} and Vojtěch {Enev} and Dan {Kučera} and Pavla {Benešová} and Stanislav {Obruča}",
title="Production and characterization of extracellular polysaccharide of extremely halophilic Archaea Haloferax mediterranei",
booktitle="6th Central European Congress of Life Sciences Eurobiotech",
year="2017",
pages="156--157",
address="Krakow",
note="Abstract"
}