Publication result detail

Optimalization of deoxyribonucleic acid extraction using variuos types of magnetic particles

KONEČNÁ, J.; ROMANOVSKÁ, D.; TRACHTOVÁ, Š.

Original Title

Optimalization of deoxyribonucleic acid extraction using variuos types of magnetic particles

English Title

Optimalization of deoxyribonucleic acid extraction using variuos types of magnetic particles

Type

WoS Article

Original Abstract

Isolation of deoxyribonucleic acid is an important step in the molecular diagnostics of microorganisms. A high quality of isolated deoxyribonucleic acid is necessary for deoxyribonucleic acid amplification by the polymerase chain reaction. The conventional deoxyribinucleic acid isolation using phenol-chloroform extraction and deoxyribonucleic acid precipitation in ethanol is time-consumingand requires the use of oxic phenol. Magnetic separation techniques using magnetic solid particles are one of modern methods to speed up the nucleic acids isolation. The ain of this work was to use two different types of deoxyribonucleic acid in separation mixtures were measured using ultraviolet spectrophotometry.

English abstract

Isolation of deoxyribonucleic acid is an important step in the molecular diagnostics of microorganisms. A high quality of isolated deoxyribonucleic acid is necessary for deoxyribonucleic acid amplification by the polymerase chain reaction. The conventional deoxyribinucleic acid isolation using phenol-chloroform extraction and deoxyribonucleic acid precipitation in ethanol is time-consumingand requires the use of oxic phenol. Magnetic separation techniques using magnetic solid particles are one of modern methods to speed up the nucleic acids isolation. The ain of this work was to use two different types of deoxyribonucleic acid in separation mixtures were measured using ultraviolet spectrophotometry.

Keywords

Magnetic particles, Solid-phase extraction, Polymerase chain reaction, Deoxyribonucleic acid isolation

Key words in English

Magnetic particles, Solid-phase extraction, Polymerase chain reaction, Deoxyribonucleic acid isolation

Authors

KONEČNÁ, J.; ROMANOVSKÁ, D.; TRACHTOVÁ, Š.

RIV year

2019

Released

01.05.2019

Publisher

Chemical papers

Location

Institute of Chemistry, Slovak Academy of Sciences

ISBN

1336-9075

Periodical

Chemical Papers

Volume

120

Number

13

State

Slovak Republic

Pages from

1

Pages to

9

Pages count

9

URL

https://link.springer.com/article/10.1007/s11696-018-00675-9

BibTex

@article{BUT151274,
  author="Jana {Konečná} and Denisa {Langová} and Štěpánka {Trachtová}",
  title="Optimalization of deoxyribonucleic acid extraction using variuos types of magnetic particles",
  journal="Chemical Papers",
  year="2019",
  volume="120",
  number="13",
  pages="1--9",
  doi="10.1007/s11696-018-00675-9",
  issn="1336-9075",
  url="https://link.springer.com/article/10.1007/s11696-018-00675-9"
}