Publication result detail
Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth
VANĚK, M.; MRAVEC, F.; SZOTKOWSKI, M.; BYRTUSOVÁ, D.; HÁRONIKOVÁ, A.; MÁROVÁ, I.
Original Title
Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth
English Title
Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth
Type
WoS Article
Original Abstract
Red yeast Cystofilobasidium capitatum autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells. Yeasts were cultivated in 2L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and HPLC determination of pigments. To compare FLIM (Fluorescence Lifetime Imaging Microscopy) images and coupled data (obtained by CLSM) with model systems, FLIM analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, and with constant addition of beta-carotene. Liposomes lecithin:ergosterol:beta-carotene were investigated too. Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoid protective system in compartments of yeast cell, especially cytoplasmic membrane.
English abstract
Red yeast Cystofilobasidium capitatum autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells. Yeasts were cultivated in 2L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and HPLC determination of pigments. To compare FLIM (Fluorescence Lifetime Imaging Microscopy) images and coupled data (obtained by CLSM) with model systems, FLIM analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, and with constant addition of beta-carotene. Liposomes lecithin:ergosterol:beta-carotene were investigated too. Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoid protective system in compartments of yeast cell, especially cytoplasmic membrane.
Keywords
autofluorescence, carotenoids, Cystofilobasidium capitatum, fluorescence lifetime imaging, red yeasts
Key words in English
autofluorescence, carotenoids, Cystofilobasidium capitatum, fluorescence lifetime imaging, red yeasts
Authors
VANĚK, M.; MRAVEC, F.; SZOTKOWSKI, M.; BYRTUSOVÁ, D.; HÁRONIKOVÁ, A.; MÁROVÁ, I.
RIV year
2019
Released
10.04.2018
Publisher
De Gruyter Open
Location
Itálie
ISBN
2564-615X
Periodical
EuroBiotech Journal
Volume
2
Number
2
State
Republic of Italy
Pages from
114
Pages to
120
Pages count
7
URL
BibTex
@article{BUT155466,
author="Martin {Vaněk} and Filip {Mravec} and Martin {Szotkowski} and Dana {Byrtusová} and Andrea {Němcová} and Ivana {Márová}",
title="Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth",
journal="EuroBiotech Journal",
year="2018",
volume="2",
number="2",
pages="114--120",
doi="10.2478/ebtj-2018-0015",
url="http://www.degruyter.com/view/j/ebtj.2018.2.issue-2/ebtj-2018-0015/ebtj-2018-0015.xml?format=INT"
}Documents