Detail publikačního výsledku
An appropriate method for assessing hydrogel pore sizes by cryo-SEM
ADÁMKOVÁ, K.; TRUDIČOVÁ, M.; SEDLÁČEK, P.; KRZYŽÁNEK, V.; HRUBANOVÁ, K.
Original Title
An appropriate method for assessing hydrogel pore sizes by cryo-SEM
English Title
An appropriate method for assessing hydrogel pore sizes by cryo-SEM
Type
Paper in proceedings (conference paper)
Original Abstract
The aim of our work was to examine and describe ultrastructure of the agarose hydrogel and any possible structural concentration dependencies, and to assess the distribution and size of pores of agarose hydrogel in dependence on its concentration. Four concentrations were prepared (0.5 %, 1.0 %, 2.0 % and 4.0 % of dry weight content) and cryo-SEM and turbidimetry methods were executed on wet (original) samples in order to image the ultrastructure and measure the pore sizes within. Reasonable results were obtained for the wet samples as they were closer to their native state they are usually used for applications in. Cryo-SEM and turbidimetry provided comparable results of pore diameters and allowed to compare pore diameters dependant on the concentrations; moreover, it showed more detailed and realistic structure.
English abstract
The aim of our work was to examine and describe ultrastructure of the agarose hydrogel and any possible structural concentration dependencies, and to assess the distribution and size of pores of agarose hydrogel in dependence on its concentration. Four concentrations were prepared (0.5 %, 1.0 %, 2.0 % and 4.0 % of dry weight content) and cryo-SEM and turbidimetry methods were executed on wet (original) samples in order to image the ultrastructure and measure the pore sizes within. Reasonable results were obtained for the wet samples as they were closer to their native state they are usually used for applications in. Cryo-SEM and turbidimetry provided comparable results of pore diameters and allowed to compare pore diameters dependant on the concentrations; moreover, it showed more detailed and realistic structure.
Keywords
hydrogel, cryo-SEM, freezing methods, agarose, image analysis
Key words in English
hydrogel, cryo-SEM, freezing methods, agarose, image analysis
Authors
ADÁMKOVÁ, K.; TRUDIČOVÁ, M.; SEDLÁČEK, P.; KRZYŽÁNEK, V.; HRUBANOVÁ, K.
RIV year
2020
Released
01.01.2019
Publisher
TANGER Ltd.
Location
Ostrava
ISBN
9788087294895
Book
NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application
Edition
1
Pages from
415
Pages to
420
Pages count
6
Full text in the Digital Library
BibTex
@inproceedings{BUT157932,
author="ADÁMKOVÁ, K. and TRUDIČOVÁ, M. and SEDLÁČEK, P. and KRZYŽÁNEK, V. and HRUBANOVÁ, K.",
title="An appropriate method for assessing hydrogel pore sizes by cryo-SEM",
booktitle="NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application",
year="2019",
series="1",
number="1",
pages="415--420",
publisher="TANGER Ltd.",
address="Ostrava",
isbn="9788087294895"
}