Publication result detail

Time evolution of trapped single cell microorganism

BERNATOVA, S.; SAMEK, O.; OBRUČA, S.; SERY, M.; ZEMANEK, P.; MÁROVÁ, I.

Original Title

Time evolution of trapped single cell microorganism

English Title

Time evolution of trapped single cell microorganism

Type

Paper in proceedings (conference paper)

Original Abstract

The combination of optical tweezers and Raman micro-spectroscopy is frequently referred as Raman tweezers. A single focused laser beam is utilized here both as a source of Raman scattering and a source forming an optical trap. Raman tweezers have been recently used in variety of applications in cell biology as a useful tool for non-contact and non-destructive determination of living cells properties. Here we use Raman tweezers to follow response of cells on the length of their cultivation in mineral oil. Analyses of obtained Raman spectra are based on 2D correlation analysis and allow us to determine the chemical background of the cell response in a gentle way.

English abstract

The combination of optical tweezers and Raman micro-spectroscopy is frequently referred as Raman tweezers. A single focused laser beam is utilized here both as a source of Raman scattering and a source forming an optical trap. Raman tweezers have been recently used in variety of applications in cell biology as a useful tool for non-contact and non-destructive determination of living cells properties. Here we use Raman tweezers to follow response of cells on the length of their cultivation in mineral oil. Analyses of obtained Raman spectra are based on 2D correlation analysis and allow us to determine the chemical background of the cell response in a gentle way.

Keywords

Raman tweezers; Raman spectroscopy; bacteria; polyhydroxyalkanoates

Key words in English

Raman tweezers; Raman spectroscopy; bacteria; polyhydroxyalkanoates

Authors

BERNATOVA, S.; SAMEK, O.; OBRUČA, S.; SERY, M.; ZEMANEK, P.; MÁROVÁ, I.

RIV year

2020

Released

01.02.2016

Publisher

SPIE

Location

San Francisco

ISBN

978-1-62841-945-0

Book

IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX

Pages from

1

Pages to

5

Pages count

5

URL

https://www.spiedigitallibrary.org/conference-proceedings-of-spie/9711/1/Time-evolution-of-trapped-single-cell-microorganism/10.1117/12.2213264.short?SSO=1

BibTex

@inproceedings{BUT163455,
  author="BERNATOVA, S. and SAMEK, O. and OBRUČA, S. and SERY, M. and ZEMANEK, P. and MÁROVÁ, I.",
  title="Time evolution of trapped single cell microorganism",
  booktitle="IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX",
  year="2016",
  pages="1--5",
  publisher="SPIE",
  address="San Francisco",
  doi="10.1117/12.2213264",
  isbn="978-1-62841-945-0",
  url="https://www.spiedigitallibrary.org/conference-proceedings-of-spie/9711/1/Time-evolution-of-trapped-single-cell-microorganism/10.1117/12.2213264.short?SSO=1"
}