Publication detail

Production of polyhydroxyalkanoates (PHA) by a thermophilic strain of Schlegelella thermodepolymerans from xylose rich substrates

KOUŘILOVÁ, X. PERNICOVÁ, I. SEDLÁŘ, K. MUSILOVÁ, J. SEDLÁČEK, P. KALINA, M. KOLLER, M. OBRUČA, S.

Original Title

Production of polyhydroxyalkanoates (PHA) by a thermophilic strain of Schlegelella thermodepolymerans from xylose rich substrates

English Title

Production of polyhydroxyalkanoates (PHA) by a thermophilic strain of Schlegelella thermodepolymerans from xylose rich substrates

Type

journal article in Web of Science

Language

en

Original Abstract

The aim of this work was to investigate the thermophilic bacterium Schelegelella thermodepolymerans DSM 15344 in terms of its polyhydroxyalkanoates (PHA) biosynthesis capacity. The bacterium is capable of converting various sugars into PHA with the optimal growth temperature of 55 °C; therefore, the process of PHA biosynthesis could be robust against contamination. Surprisingly, the highest yield was gained on xylose. Results suggested that S. thermodepolymerans possess unique xylose metabolism since xylose is utilized preferentially with the highest consumption rate as compared to other sugars. In the genome of S. thermodepolymerans DSM 15344, a unique putative xyl operon consisting of genes responsible for xylose utilization and also for its transport was identified, which is a unique feature among PHA producers. The bacterium is capable of biosynthesis of copolymers containing 3-hydroxybutyrate and also 3-hydroxyvalerate subunits. Hence, S. thermodepolymerans seems to be promising candidate for PHA production from xylose rich substrates.

English abstract

The aim of this work was to investigate the thermophilic bacterium Schelegelella thermodepolymerans DSM 15344 in terms of its polyhydroxyalkanoates (PHA) biosynthesis capacity. The bacterium is capable of converting various sugars into PHA with the optimal growth temperature of 55 °C; therefore, the process of PHA biosynthesis could be robust against contamination. Surprisingly, the highest yield was gained on xylose. Results suggested that S. thermodepolymerans possess unique xylose metabolism since xylose is utilized preferentially with the highest consumption rate as compared to other sugars. In the genome of S. thermodepolymerans DSM 15344, a unique putative xyl operon consisting of genes responsible for xylose utilization and also for its transport was identified, which is a unique feature among PHA producers. The bacterium is capable of biosynthesis of copolymers containing 3-hydroxybutyrate and also 3-hydroxyvalerate subunits. Hence, S. thermodepolymerans seems to be promising candidate for PHA production from xylose rich substrates.

Keywords

Schlegelella thermodepolymerans; Polylyhydroxyalkanoates; Thermophiles; Xylose

Released

20.08.2020

Publisher

Elsevier

Pages from

1

Pages to

7

Pages count

7

URL

Documents

BibTex


@article{BUT164767,
  author="Xenie {Kouřilová} and Iva {Pernicová} and Karel {Sedlář} and Jana {Musilová} and Petr {Sedláček} and Michal {Kalina} and Stanislav {Obruča}",
  title="Production of polyhydroxyalkanoates (PHA) by a thermophilic strain of Schlegelella thermodepolymerans from xylose rich substrates",
  annote="The aim of this work was to investigate the thermophilic bacterium Schelegelella thermodepolymerans DSM 15344 in terms of its polyhydroxyalkanoates (PHA) biosynthesis capacity. The bacterium is capable of converting various sugars into PHA with the optimal growth temperature of 55 °C; therefore, the process of PHA biosynthesis could be robust against contamination. Surprisingly, the highest yield was gained on xylose. Results suggested that S. thermodepolymerans possess unique xylose metabolism since xylose is utilized preferentially with the highest consumption rate as compared to other sugars. In the genome of S. thermodepolymerans DSM 15344, a unique putative xyl operon consisting of genes responsible for xylose utilization and also for its transport was identified, which is a unique feature among PHA producers. The bacterium is capable of biosynthesis of copolymers containing 3-hydroxybutyrate and also 3-hydroxyvalerate subunits. Hence, S. thermodepolymerans seems to be promising candidate for PHA production from xylose rich substrates.",
  address="Elsevier",
  chapter="164767",
  doi="10.1016/j.biortech.2020.123885",
  howpublished="online",
  institution="Elsevier",
  number="8",
  volume="315",
  year="2020",
  month="august",
  pages="1--7",
  publisher="Elsevier",
  type="journal article in Web of Science"
}