Detail publikačního výsledku
Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil
POSPÍŠILOVÁ, A.; NOVÁČKOVÁ, I.; PŘIKRYL, R.
Original Title
Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil
English Title
Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil
Type
WoS Article
Original Abstract
The aim of this work was to develop a soap-based method for the isolation of poly(3-hydroxybutyrate) from bacterial biomass. The method consisted of adding soap derived from waste cooking oil to a concentrated (25%) biomass suspension, heating and centrifugal separation. Purity above 95% could be achieved with soap:cell dry mass ratios at least 0.125 g/g, making the method comparable to other surfactant-based protocols. Molecular weights M, of products from all experiments were between 350 and 450 kDa, being high enough for future material applications. Addition of hydrochloric acid to the wastewater led to the precipitation of soap and part of non-P3HB cell mass. The resulting precipitate was utilized as a carbon source in biomass production and increased substrate-to-P3HB conversion.
English abstract
The aim of this work was to develop a soap-based method for the isolation of poly(3-hydroxybutyrate) from bacterial biomass. The method consisted of adding soap derived from waste cooking oil to a concentrated (25%) biomass suspension, heating and centrifugal separation. Purity above 95% could be achieved with soap:cell dry mass ratios at least 0.125 g/g, making the method comparable to other surfactant-based protocols. Molecular weights M, of products from all experiments were between 350 and 450 kDa, being high enough for future material applications. Addition of hydrochloric acid to the wastewater led to the precipitation of soap and part of non-P3HB cell mass. The resulting precipitate was utilized as a carbon source in biomass production and increased substrate-to-P3HB conversion.
Keywords
PHA; P3HB; Recovery; Downstream process; Isolation
Key words in English
PHA; P3HB; Recovery; Downstream process; Isolation
Authors
POSPÍŠILOVÁ, A.; NOVÁČKOVÁ, I.; PŘIKRYL, R.
RIV year
2021
Released
01.04.2021
Publisher
Elsevier
Location
OXFORD
ISBN
0960-8524
Periodical
BIORESOURCE TECHNOLOGY
Volume
326
Number
1
State
Kingdom of the Netherlands
Pages from
1
Pages to
5
Pages count
5
URL
Full text in the Digital Library
BibTex
@article{BUT171646,
author="Aneta {Pospíšilová} and Ivana {Nováčková} and Radek {Přikryl}",
title="Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil",
journal="BIORESOURCE TECHNOLOGY",
year="2021",
volume="326",
number="1",
pages="1--5",
doi="10.1016/j.biortech.2021.124683",
issn="0960-8524",
url="https://www.sciencedirect.com/science/article/pii/S0960852421000213"
}Documents