Publication detail

PHA co-polymers production in two cyanobacterial strains from Synechocystis family

ŠEDRLOVÁ, Z. SLANINOVÁ, E. OBRUČA, S.

Original Title

PHA co-polymers production in two cyanobacterial strains from Synechocystis family

Type

abstract

Language

English

Original Abstract

Cyanobacteria are ecologically extremely important phototrophic gram-negative which belong to the prokaryotes, cyanobacteria contains plastids so they capable to do oxygenic photosynthesis. Cyanobacteria synthetize many interesting metabolites such as glycogen, lipids, carotenoids, but the most interesting metabolites are polyhydroxyalkanoates (PHA). PHA are biodegradable biopolymers with similar properties as petrochemical plastics and they occure in bacteria in granules. Numerous prokaryotes including cyanobacteria use PHA as a storage of carbon and energy in form of intracellular granules. The most common type of PHA is poly-3-hydroxybutyrate (PHB). PHA can be produced by heterotrophic bacteria as well, such as Cupriavidus necator H16 or Methylobacterium rhodesianum. PHB raises fitness of bacteria because it helps the bacteria to survive the stress conditions – osmotic stress, UV radiation, freezing or high temperatures. Both strains are capable of co-polymer synthesis in presence of its structural precursor. The composition of co-polymer differs in Synechocystis sp. PCC 6803 and Synechocystis salina CCALA 192 and the composition of co-polymers also varies depending on the precursor concentration. Synechocystis sp. PCC 6803 and CCALA 192 cultivated with acetate and γ-butyrolakton accumulated co-polymer of 3-hydroxybutyrate (3HB) and 4-hydroxybutyrate (4HB). 4HB was for the first time in cyanobacteria reported in Synechococcus sp. PCC 7002 in 2015 an in PCC 6803 in 2020. The total amount of PHA as well as yield of biomass was lower than in a control due to the toxic properties of γ-butyrolakton.

Keywords

Cyanobacteria, co-polymer, Synechocystis

Authors

ŠEDRLOVÁ, Z.; SLANINOVÁ, E.; OBRUČA, S.

Released

28. 4. 2022

Publisher

MASARYK UNIVERSITY PRESS

Location

Brno

ISBN

978-80-280-0040-0

Book

The Biomania Student Scientific Meeting

Edition

1

Pages from

139

Pages to

139

Pages count

150

BibTex

@misc{BUT177970,
  author="Zuzana {Šedrlová} and Eva {Slaninová} and Stanislav {Obruča}",
  title="PHA co-polymers production in two cyanobacterial strains from Synechocystis family",
  booktitle="The Biomania Student Scientific Meeting",
  year="2022",
  series="1",
  pages="139--139",
  publisher="MASARYK UNIVERSITY PRESS",
  address="Brno",
  isbn="978-80-280-0040-0",
  note="abstract"
}