R&D result detail

Original Title

Morphological Study of PHA Producing Bacteria

English Title

Morphological Study of PHA Producing Bacteria

Type

Paper in proceedings (conference paper)

Original Abstract

Our comprehensive morphological study indicates that the method involving cryo-SEM imaging in combination with highpressure HPF freezing and perpendicular freeze-fracturing, is an excellent technique for analysing highly hydrated samples. Cells containing polymer particles, which are elastic even at very low temperatures, were visualized using this method. By TEM it is able to monitor the distribution of PHB granules inside the microbial cells. SBFSEM provides spatial information. A comparison of the morphology of the internal and external cell structure shows that the Cupriavidus necator H16 contains significant amounts of PHA granules, which are distributed throughout the whole cell volume .

English abstract

Our comprehensive morphological study indicates that the method involving cryo-SEM imaging in combination with highpressure HPF freezing and perpendicular freeze-fracturing, is an excellent technique for analysing highly hydrated samples. Cells containing polymer particles, which are elastic even at very low temperatures, were visualized using this method. By TEM it is able to monitor the distribution of PHB granules inside the microbial cells. SBFSEM provides spatial information. A comparison of the morphology of the internal and external cell structure shows that the Cupriavidus necator H16 contains significant amounts of PHA granules, which are distributed throughout the whole cell volume .

Keywords

polyhydroxyalkanoates; electron microscopy; Cupriavidus necator

Key words in English

polyhydroxyalkanoates; electron microscopy; Cupriavidus necator

Authors

HRUBANOVÁ, K.; SIKOROVÁ, P.; MRÁZOVÁ, K.; NEBESÁŘOVÁ, J.; OBRUČA, S.; KRZYŽÁNEK, V.

RIV year

2024

Released

31.10.2023

Publisher

Oxford Academic

Location

Oxford

Book

Mictoscopy and Microanalysis

ISBN

1435-8115

Periodical

MICROSCOPY AND MICROANALYSIS

Volume

29

Number

1

State

United States of America

Pages from

883

Pages to

884

Pages count

2

URL

https://watermark.silverchair.com/ozad067.436.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA24wggNqBgkqhkiG9w0BBwagggNbMIIDVwIBADCCA1AGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM6zvKSZpfNQkegG0tAgEQgIIDIdKmjtZo1JTIbtb6RFZkFuVEwyCH50hW3BGT1LvIQEWQLtR2vrZuSYNt0-RFn4X-My459ZXHgDgT3rXVyN2hr5IheLy7h3coAB9uWNHZyMpECgDPA-kncAOzVulTsU3Kjj0NO5WEeWIPn_AD_PzvxEI3tKdoFX0tkT_Mig2zipqdYSafxFl9M7Eo3l-paA0fN24q4yb7KIe_uNS4DIL9MK9qy-r74U0ObIq4W00RuFrCimenl2YzSQ9e8U42dFsaNmZ9C7mod63Zd98fCNbOydwTwq9_puW6rJVG2OY7jDWF

BibTex

@inproceedings{BUT187572,
  author="Kamila {Hrubanová} and Pavlína {Sikorová} and Kateřina {Mrázová} and Jana {Nebesářová} and Stanislav {Obruča} and Vladislav {Krzyžánek}",
  title="Morphological Study of PHA Producing Bacteria",
  booktitle="Mictoscopy and Microanalysis",
  year="2023",
  journal="MICROSCOPY AND MICROANALYSIS",
  volume="29",
  number="1",
  pages="883--884",
  publisher="Oxford Academic",
  address="Oxford",
  doi="10.1093/micmic/ozad067.436",
  issn="1431-9276",
  url="https://watermark.silverchair.com/ozad067.436.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA24wggNqBgkqhkiG9w0BBwagggNbMIIDVwIBADCCA1AGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM6zvKSZpfNQkegG0tAgEQgIIDIdKmjtZo1JTIbtb6RFZkFuVEwyCH50hW3BGT1LvIQEWQLtR2vrZuSYNt0-RFn4X-My459ZXHgDgT3rXVyN2hr5IheLy7h3coAB9uWNHZyMpECgDPA-kncAOzVulTsU3Kjj0NO5WEeWIPn_AD_PzvxEI3tKdoFX0tkT_Mig2zipqdYSafxFl9M7Eo3l-paA0fN24q4yb7KIe_uNS4DIL9MK9qy-r74U0ObIq4W00RuFrCimenl2YzSQ9e8U42dFsaNmZ9C7mod63Zd98fCNbOydwTwq9_puW6rJVG2OY7jDWF"
}