Publication result detail
Determination of mycotoxins and their dietary exposure assessment in pale lager beers using immunoaffinity columns and UPLC-MS/MS
SVOBODA, Z.; BĚLÁKOVÁ, S.
Original Title
Determination of mycotoxins and their dietary exposure assessment in pale lager beers using immunoaffinity columns and UPLC-MS/MS
English Title
Determination of mycotoxins and their dietary exposure assessment in pale lager beers using immunoaffinity columns and UPLC-MS/MS
Type
WoS Article
Original Abstract
The use of contaminated raw materials can lead to the transfer of mycotoxins into the final product, including beer. This study describes the use of the commercially available immunoaffinity column 11+Myco MS-PREP® and UPLC-MS/MS for the determination of mycotoxins in pale lager-type beers brewed in Czech Republic and other European countries. The additional aim of the work was to develop, optimize and validate this analytical method. Validation parameters such as linearity, limit of detection (LOD), limit of quantification (LOQ), precision and accuracy were tested. The calibration curves were linear with correlation coefficients (R2 > 0.99) for all mycotoxins under investigation. The LOD ranged from 0.1 to 50 ng/L and LOQ from 0.4 to 167 ng/L. Recoveries of the selected analytes ranged from 72.2 to 101.1%, and the relative standard deviation under conditions repeatability (RSDr) did not exceed 16.3% for any mycotoxin. The validated procedure was successfully applied for the analysis of mycotoxins in a total of 89 beers from the retail network. The results were also processed using advanced chemometric techniques and compared with similar published studies. The toxicological impact was taken into account.
English abstract
The use of contaminated raw materials can lead to the transfer of mycotoxins into the final product, including beer. This study describes the use of the commercially available immunoaffinity column 11+Myco MS-PREP® and UPLC-MS/MS for the determination of mycotoxins in pale lager-type beers brewed in Czech Republic and other European countries. The additional aim of the work was to develop, optimize and validate this analytical method. Validation parameters such as linearity, limit of detection (LOD), limit of quantification (LOQ), precision and accuracy were tested. The calibration curves were linear with correlation coefficients (R2 > 0.99) for all mycotoxins under investigation. The LOD ranged from 0.1 to 50 ng/L and LOQ from 0.4 to 167 ng/L. Recoveries of the selected analytes ranged from 72.2 to 101.1%, and the relative standard deviation under conditions repeatability (RSDr) did not exceed 16.3% for any mycotoxin. The validated procedure was successfully applied for the analysis of mycotoxins in a total of 89 beers from the retail network. The results were also processed using advanced chemometric techniques and compared with similar published studies. The toxicological impact was taken into account.
Keywords
11+Myco MS-PREP®; Beer; Dietary exposure; Immunoaffinity columns; Mycotoxins; UPLC-MS/MS
Key words in English
11+Myco MS-PREP®; Beer; Dietary exposure; Immunoaffinity columns; Mycotoxins; UPLC-MS/MS
Authors
SVOBODA, Z.; BĚLÁKOVÁ, S.
RIV year
2024
Released
19.06.2023
Publisher
Springer
ISBN
1867-1632
Periodical
Mycotoxin Research
Volume
39
Number
August
State
Federal Republic of Germany
Pages from
285
Pages to
302
Pages count
8
URL
Full text in the Digital Library
BibTex
@article{BUT188057,
author="Zdeněk {Svoboda} and Silvie {Běláková}",
title="Determination of mycotoxins and their dietary exposure assessment in pale lager beers using immunoaffinity columns and UPLC-MS/MS",
journal="Mycotoxin Research",
year="2023",
volume="39",
number="August",
pages="285--302",
doi="10.1007/s12550-023-00492-4",
issn="0178-7888",
url="https://link.springer.com/article/10.1007/s12550-023-00492-4#article-info"
}