R&D result detail

Original Title

Determination of mycotoxins and their dietary exposure assessment in pale lager beers using immunoaffinity columns and UPLC-MS/MS

English Title

Determination of mycotoxins and their dietary exposure assessment in pale lager beers using immunoaffinity columns and UPLC-MS/MS

Type

WoS Article

Original Abstract

The use of contaminated raw materials can lead to the transfer of mycotoxins into the final product, including beer. This study describes the use of the commercially available immunoaffinity column 11+Myco MS-PREP® and UPLC-MS/MS for the determination of mycotoxins in pale lager-type beers brewed in Czech Republic and other European countries. The additional aim of the work was to develop, optimize and validate this analytical method. Validation parameters such as linearity, limit of detection (LOD), limit of quantification (LOQ), precision and accuracy were tested. The calibration curves were linear with correlation coefficients (R2 > 0.99) for all mycotoxins under investigation. The LOD ranged from 0.1 to 50 ng/L and LOQ from 0.4 to 167 ng/L. Recoveries of the selected analytes ranged from 72.2 to 101.1%, and the relative standard deviation under conditions repeatability (RSDr) did not exceed 16.3% for any mycotoxin. The validated procedure was successfully applied for the analysis of mycotoxins in a total of 89 beers from the retail network. The results were also processed using advanced chemometric techniques and compared with similar published studies. The toxicological impact was taken into account.

English abstract

The use of contaminated raw materials can lead to the transfer of mycotoxins into the final product, including beer. This study describes the use of the commercially available immunoaffinity column 11+Myco MS-PREP® and UPLC-MS/MS for the determination of mycotoxins in pale lager-type beers brewed in Czech Republic and other European countries. The additional aim of the work was to develop, optimize and validate this analytical method. Validation parameters such as linearity, limit of detection (LOD), limit of quantification (LOQ), precision and accuracy were tested. The calibration curves were linear with correlation coefficients (R2 > 0.99) for all mycotoxins under investigation. The LOD ranged from 0.1 to 50 ng/L and LOQ from 0.4 to 167 ng/L. Recoveries of the selected analytes ranged from 72.2 to 101.1%, and the relative standard deviation under conditions repeatability (RSDr) did not exceed 16.3% for any mycotoxin. The validated procedure was successfully applied for the analysis of mycotoxins in a total of 89 beers from the retail network. The results were also processed using advanced chemometric techniques and compared with similar published studies. The toxicological impact was taken into account.

Keywords

11+Myco MS-PREP®; Beer; Dietary exposure; Immunoaffinity columns; Mycotoxins; UPLC-MS/MS

Key words in English

11+Myco MS-PREP®; Beer; Dietary exposure; Immunoaffinity columns; Mycotoxins; UPLC-MS/MS

Authors

SVOBODA, Z.; BĚLÁKOVÁ, S.

RIV year

2024

Released

19.06.2023

Publisher

Springer

ISBN

1867-1632

Periodical

Mycotoxin Research

Volume

39

Number

August

State

Federal Republic of Germany

Pages from

285

Pages to

302

Pages count

8

URL

https://link.springer.com/article/10.1007/s12550-023-00492-4#article-info

BibTex

@article{BUT188057,
  author="Zdeněk {Svoboda} and Silvie {Běláková}",
  title="Determination of mycotoxins and their dietary exposure assessment in pale lager beers using immunoaffinity columns and UPLC-MS/MS",
  journal="Mycotoxin Research",
  year="2023",
  volume="39",
  number="August",
  pages="285--302",
  doi="10.1007/s12550-023-00492-4",
  issn="0178-7888",
  url="https://link.springer.com/article/10.1007/s12550-023-00492-4#article-info"
}