Publication detail

Upcycling atmospheric CO2 to polyhydroxyalkanoates via sequential chemo-biocatalytic processes

By Bruch, M. Sanchez-Velandia, JE. Rodriguez-Pereira, J. Rich, M. Pearcy, N. Narancic, T. Garcia-Verdugo, E. Sans, V. O'Connor, K. Zanatta, M

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

The reduction of greenhouse gas emissions and the shift away from petrochemical-derived materials are critical goals in modern industrial development and societal progress. Addressing these intertwined challenges demands innovative and sustainable solutions. Here, we present the first example of synthesizing poly[R-(-)-3-hydroxybutyrate] (PHB) from atmospheric CO2, utilizing a streamlined and integrated process that combines both chemo- and bio-catalytic conditions. Central to our approach is the development of an immobilized catalytic system that efficiently converts atmospheric CO2 into sodium formate, establishing a sustainable carbon source for formatotrophic organisms. Through Adaptive Laboratory Evolution (ALE), we enhanced the growth rate of the bacterium Cupriavidus necator H16, enabling it to utilize formic acid and formate as the sole carbon and energy sources. The evolved strain, C. necator ALE26, achieved a 1.8-fold increase in the maximum growth rate (mu(max) = 0.25 +/- 0.02 h(-1)), attributed to the loss of the megaplasmid pHG1. Employing the adapted strain, we report the highest PHB production rate in continuous fermentation using C. necator for growth on formate. The development of the different stages (sorption and chemo- and bio-transformation) under compatible conditions that minimize the number of work-up stages demonstrates a major advancement in converting atmospheric CO2 into valuable biopolymers, thus simultaneously contributing to the reduction of greenhouse gases in the atmosphere and to a circular economy of biobased polymers that diminish fossil fuel dependence.

Keywords

FORMIC-ACID, RALSTONIA-EUTROPHA, CARBON-DIOXIDE

Authors

By Bruch, M.; Sanchez-Velandia, JE.; Rodriguez-Pereira, J.; Rich, M.; Pearcy, N.; Narancic, T.; Garcia-Verdugo, E.; Sans, V.; O'Connor, K.; Zanatta, M

Released

9. 12. 2024

Publisher

ROYAL SOC CHEMISTRY

Location

CAMBRIDGE

ISBN

1463-9262

Periodical

GREEN CHEMISTRY

Year of study

Number

State

United Kingdom of Great Britain and Northern Ireland

Pages from

11885

Pages to

1189

Pages count

URL

https://pubs.rsc.org/en/content/articlelanding/2024/gc/d4gc04228j

BibTex

@article{BUT191210,
  author="Jhonatan {Rodriguez Pereira} and Manuel {Bruch} and Julian E. {Sanchez-Velandia, Julian E.} and Michelle {Rich} and Nicole {Pearcy} and Tanja {Narančić} and Eduardo {Garcia-Verdugo} and Víctor {Sans} and Kevin {O'Connor} and Marcileia {Zanatta}",
  title="Upcycling atmospheric CO2 to polyhydroxyalkanoates via sequential chemo-biocatalytic processes",
  journal="GREEN CHEMISTRY",
  year="2024",
  volume="26",
  number="24",
  pages="11885--1189",
  doi="10.1039/d4gc04228j",
  issn="1463-9262",
  url="https://pubs.rsc.org/en/content/articlelanding/2024/gc/d4gc04228j"
}