OPTIMIZATION OF EXTRACTION, PURIFICATION AND STABILIZATION OF BIOLOGICALLY ACTIVE COMPOUNDS FROM YEAST AND MICROALGAE BIOMASS

OPTIMIZATION OF EXTRACTION, PURIFICATION AND STABILIZATION OF BIOLOGICALLY ACTIVE COMPOUNDS FROM YEAST AND MICROALGAE BIOMASS

Paper in proceedings (conference paper)

The rapid growth of biotechnological industry is caused by the flexible capabilities of microorganisms, such as their ability to turn waste materials into valuable compounds. Some of these compounds can be used as vitamin precursors or biofuel sources. The topic of this research is focused on the optimisation of extraction of valuable lipophilic compounds from red yeast biomass and microalgae biomass. Solvents used should be suitable for food or pharmaceutical industry, in particular ethanol and hexane. The stability of prepared extracts, while stored at various ambient temperatures, is also tested. Cultivation of these microorganisms includes small-scale fermenter cultivations and cultivations in Pyrex Glass Bottles with profound aeration. Generally, ethanol proved to be better extraction solvent, when it comes to extraction of pigments than hexane. However, hexane seems to be more suitable for extraction of lipids. Also, it is worth noting, there have been observed some specific biomass samples, for which both solvents proved to be comparably efficient. The prepared extracts exhibited good stability while stored in the freezer and only some of them proved to be stable in the fridge. Compounds in almost all extracts quickly deteriorated, while stored at laboratory temperatures.

The rapid growth of biotechnological industry is caused by the flexible capabilities of microorganisms, such as their ability to turn waste materials into valuable compounds. Some of these compounds can be used as vitamin precursors or biofuel sources. The topic of this research is focused on the optimisation of extraction of valuable lipophilic compounds from red yeast biomass and microalgae biomass. Solvents used should be suitable for food or pharmaceutical industry, in particular ethanol and hexane. The stability of prepared extracts, while stored at various ambient temperatures, is also tested. Cultivation of these microorganisms includes small-scale fermenter cultivations and cultivations in Pyrex Glass Bottles with profound aeration. Generally, ethanol proved to be better extraction solvent, when it comes to extraction of pigments than hexane. However, hexane seems to be more suitable for extraction of lipids. Also, it is worth noting, there have been observed some specific biomass samples, for which both solvents proved to be comparably efficient. The prepared extracts exhibited good stability while stored in the freezer and only some of them proved to be stable in the fridge. Compounds in almost all extracts quickly deteriorated, while stored at laboratory temperatures.

Extraction, puification, stabilization, yeasts, microalgae

Extraction, puification, stabilization, yeasts, microalgae

HOLUB, J.; OBRAČAJ, J.; DZURICKÁ, L.; MÁROVÁ, I.

2025

01.11.2024

CZECH SOC INDUSTRIAL CHEMISTRYNOVOTNEHO LAVKA 5, PRAGUE, 116 68, CZECH REPUBLIC

Praha

Proceedings Papers, 9th International Conference on Chemical Technology (ICCT)

první

2336-8128

International Conference on Chemical Technology

1

1

Czech Republic

378

381

4

https://www.webofscience.com/wos/woscc/full-record/WOS:001242890900064

http://hdl.handle.net/