Publication detail

Self-entrapment of Azotobacter vinelandii cultures by gelation of their exopolysaccharides: A way towards next-generation bioinoculants

ČERNAYOVÁ, D. SÚKENÍK, M. OBRUČA, S. SMILEK, J. KALINA, M. MRÁZOVÁ, K. HRUBANOVÁ, K. KRZYŽÁNEK, V. SEDLÁČEK, P.

Original Title

Self-entrapment of Azotobacter vinelandii cultures by gelation of their exopolysaccharides: A way towards next-generation bioinoculants

Type

journal article in Web of Science

Language

English

Original Abstract

Encapsulation of Plant Growth-Promoting Rhizobacteria (PGPR) in hydrogel carriers is a cutting-edge approach in developing agricultural bioinoculants, aiming to improve soil fertility and crop yield. Hydrogels provide protection against environmental stress, though traditional methods using external gel-forming agents limit economic viability. This study presents a novel approach, demonstrating the entrapment of Azotobacter vinelandii, a promising PGPR, within a gel matrix formed by Ca2+-induced crosslinking of its own exopolysaccharide, alginate. Among the five strains evaluated, A. vinelandii DSM 87, DSM 720, and DSM 13529 showed the highest alginate production, peaking at 4.9 +/- 0.6 g/L, 3.5 +/- 0.5 g/L, 3.8 +/- 0.8 g/L, and enabling stable gel formation by the fourth day of cultivation. These strains also exhibited molecular weights and chemical structures of alginate suitable for effective gelation upon Ca2+addition. Additionally, these strains demonstrated significant plant growth-promoting activities, including indole acetic acid production (up to 10.5 mu g/mL), siderophore release, and phosphate solubilization, further validating their potential for sustainable bioinoculant production. Finally, the viability of the A.vinelandii cells released from the gels was experimentally verified. Our findings support a feasible, cost-effective method for bioinoculant production that leverages A. vinelandii's intrinsic capabilities, offering a sustainable alternative to conventional agricultural practices.

Keywords

Azotobacter vinelandii; Alginate; Polyhydroxyalkanoates; Hydrogels; Bioinoculants

Authors

ČERNAYOVÁ, D.; SÚKENÍK, M.; OBRUČA, S.; SMILEK, J.; KALINA, M.; MRÁZOVÁ, K.; HRUBANOVÁ, K.; KRZYŽÁNEK, V.; SEDLÁČEK, P.

Released

17. 4. 2025

Publisher

ELSEVIER SCI LTD

Location

London

ISBN

0144-8617

Periodical

Carbohydrate Polymers

Year of study

360

Number

C

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

11

Pages count

11

URL

https://www.sciencedirect.com/science/article/pii/S0144861725003881

BibTex

@article{BUT197917,
  author="Diana {Černayová} and Martin {Súkeník} and Stanislav {Obruča} and Jiří {Smilek} and Michal {Kalina} and Kateřina {Mrázová} and Kamila {Hrubanová} and Vladislav {Krzyžánek} and Petr {Sedláček}",
  title="Self-entrapment of Azotobacter vinelandii cultures by gelation of their exopolysaccharides: A way towards next-generation bioinoculants",
  journal="Carbohydrate Polymers",
  year="2025",
  volume="360",
  number="C",
  pages="1--11",
  doi="10.1016/j.carbpol.2025.123607",
  issn="0144-8617",
  url="https://www.sciencedirect.com/science/article/pii/S0144861725003881"
}