Publication detail

Isolating biologically active β-glucans from the red yeast Rhodotorula toruloides

GERSPITZEROVÁ, N. HOLUB, J. ŠPAČKOVÁ, D. OBRAČAJ, J. MIKULÍKOVÁ, R. MÁROVÁ, I.

Original Title

Isolating biologically active β-glucans from the red yeast Rhodotorula toruloides

Type

abstract

Language

English

Original Abstract

β-glucans are polysaccharides known for their diverse biological activities, particularly their immunostimulatory properties, making them promising candidates for various therapeutic applications. The primary focus has been on β-glucans derived from150 mushrooms. However, yeast could serve as a viable alternative source offering several advantages, including higher and more consistent yields and simpler extraction processes. Nevertheless, the production of yeast-derived β-glucans remains understudied. It is essential to further optimize the extraction process for ensuring the most cost-effective production. Several isolation procedures of β-glucans from the yeast strain Rhodotorula toruloides were evaluated. Namely, alkali-acid extraction method with either harsher or milder conditions with or without subsequent lipid removal using organic solvents. The glucan content was determined using enzymatic kit. The bioactive triple-helical conformation of β-glucan extracts was verified using Congo red dye. The alkali-acid extraction method with milder conditions and further removal of the lipid content, appeared to be the most suitable isolation procedure for glucans in this specific yeast strain. The polysaccharide content reached up to 64 % (w/w) of which 78 % accounted for β-glucans. The organic solvent step was able to remove 96 % (w/w) of the present lipids. Furthermore, it was proven that the final β-glucan extract maintained its native triple-helical structure. This research contributes to a more thorough understanding of possible isolation methods of glucans in red yeast, leading to a high yield while preserving their native bioactive tertiary structure.

Keywords

glucans; yeast; isolation; Rhodotorula

Authors

GERSPITZEROVÁ, N.; HOLUB, J.; ŠPAČKOVÁ, D.; OBRAČAJ, J.; MIKULÍKOVÁ, R.; MÁROVÁ, I.

Released

14. 4. 2025

Publisher

AMCA, spol. s r.o.

Location

Praha

ISBN

978-80-88307-24-2

Book

Book of abstracts of the 12th International Conference on Chemical Technology

Edition

1

Pages from

150

Pages to

151

Pages count

2

URL

https://www.icct.cz/cs/Amca-ICCT/media/content/2025/Program/Book_of_abstracts_-ICCT2025.pdf

BibTex

@misc{BUT198375,
  author="Nela {Gerspitzerová} and Jiří {Holub} and Dominika {Špačková} and Jan {Obračaj} and Renata {Mikulíková} and Ivana {Márová}",
  title="Isolating biologically active β-glucans from the red yeast Rhodotorula toruloides",
  booktitle="Book of abstracts of the 12th International Conference on Chemical Technology",
  year="2025",
  series="1",
  pages="150--151",
  publisher="AMCA, spol. s r.o.",
  address="Praha",
  isbn="978-80-88307-24-2",
  url="https://www.icct.cz/cs/Amca-ICCT/media/content/2025/Program/Book_of_abstracts_-ICCT2025.pdf",
  note="abstract"
}