Publication result detail
Identification of viable lactobacillus cell in fermented dairy products
TRACHTOVÁ, Š.
Original Title
Identification of viable lactobacillus cell in fermented dairy products
English Title
Identification of viable lactobacillus cell in fermented dairy products
Type
Paper in proceedings (conference paper)
Original Abstract
PCR in combination with ethidium monoazidem (EMA) is a new method that allows to distinguish between live and dead cells. Expand the use of PCR in food for example when determining the number of live and dead cells of probiotic bacteria in dairy products. The method is based on the observation that EMA can penetrate into the cytoplasm and the DNA of cells killed due to malfunction of the cell membrane (wall). Photoactivation with EMA covalently binds to DNA, which is why Does Not Amplify in PCR. The PCR amplified only the DNA of living cells. The aim was to use EMA-PCR method to distinguish between live and dead bacterial cells probiotic Lactobacillus paracasei ssp paracasei CCDM 211 / 06, newly isolated from human sources. Attention was paid to various procedures of killing bacterial cells.
English abstract
PCR in combination with ethidium monoazidem (EMA) is a new method that allows to distinguish between live and dead cells. Expand the use of PCR in food for example when determining the number of live and dead cells of probiotic bacteria in dairy products. The method is based on the observation that EMA can penetrate into the cytoplasm and the DNA of cells killed due to malfunction of the cell membrane (wall). Photoactivation with EMA covalently binds to DNA, which is why Does Not Amplify in PCR. The PCR amplified only the DNA of living cells. The aim was to use EMA-PCR method to distinguish between live and dead bacterial cells probiotic Lactobacillus paracasei ssp paracasei CCDM 211 / 06, newly isolated from human sources. Attention was paid to various procedures of killing bacterial cells.
Keywords
ethidium monoazide, viable and dead bacterial cells
Key words in English
ethidium monoazide, viable and dead bacterial cells
Authors
TRACHTOVÁ, Š.
RIV year
2013
Released
20.04.2010
Publisher
ČZU v Praze
Location
Praha
ISBN
978-80-213-2072-7
Book
Sborník přednášek V. sympozia společnosti pro probiotika a prebiotika
Pages from
16
Pages to
16
Pages count
1
Full text in the Digital Library
BibTex
@inproceedings{BUT33626,
author="Štěpánka {Trachtová}",
title="Identification of viable lactobacillus cell in fermented dairy products",
booktitle="Sborník přednášek V. sympozia společnosti pro probiotika a prebiotika",
year="2010",
number="1",
pages="16--16",
publisher="ČZU v Praze",
address="Praha",
isbn="978-80-213-2072-7"
}