Detail publikačního výsledku
Identification of viable Lactobacillus cells in fermented dairy products
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.
Original Title
Identification of viable Lactobacillus cells in fermented dairy products
English Title
Identification of viable Lactobacillus cells in fermented dairy products
Type
Peer-reviewed article not indexed in WoS or Scopus
Original Abstract
The combination PCR and ethidium monoazide (EMA-PCR) is a new metod for selective distinction between viable and dead bacterial cells. The general application of EMA is based on penetration into 'dead' cells with compromised membráně (cell wall systems) integrity. DNA bound to EMA cannot be PCR amplified. Live cells remain intact and only DNA from viable cells can be amplified. Experiments were performed using probiotic bacterial strain Lactobacillus paracasei subsp. paracasei CCDM 211/06 of human origin. The experimental conditions (time of cell incubation with EMA, and cell irradiation) were optimized. In the next step instead for live-dead cell distinction propidium monoazide was used. The Lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead cells identification in milk product (yoghurt).
English abstract
The combination PCR and ethidium monoazide (EMA-PCR) is a new metod for selective distinction between viable and dead bacterial cells. The general application of EMA is based on penetration into 'dead' cells with compromised membráně (cell wall systems) integrity. DNA bound to EMA cannot be PCR amplified. Live cells remain intact and only DNA from viable cells can be amplified. Experiments were performed using probiotic bacterial strain Lactobacillus paracasei subsp. paracasei CCDM 211/06 of human origin. The experimental conditions (time of cell incubation with EMA, and cell irradiation) were optimized. In the next step instead for live-dead cell distinction propidium monoazide was used. The Lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead cells identification in milk product (yoghurt).
Keywords
ethidium monoazide, viable and dead bacterial cells
Key words in English
ethidium monoazide, viable and dead bacterial cells
Authors
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.
RIV year
2010
Released
09.09.2008
Publisher
Chemické listy
Location
Brno
ISBN
1213-7103
Periodical
CHEMICKE LISTY
Volume
102
Number
15
State
Czech Republic
Pages from
805
Pages to
806
Pages count
2
Full text in the Digital Library
BibTex
@article{BUT46735,
author="Štěpánka {Trachtová} and Alena {Španová} and Bohuslav {Rittich}",
title="Identification of viable Lactobacillus cells in fermented dairy products",
journal="CHEMICKE LISTY",
year="2008",
volume="102",
number="15",
pages="805--806",
issn="0009-2770"
}