Optimalization of Cultivation Media and Hydroxylase Enzyme Production by Aureobasidium Pullulans

Optimalization of Cultivation Media and Hydroxylase Enzyme Production by Aureobasidium Pullulans

Peer-reviewed article not indexed in WoS or Scopus

The present paper aims to study the influence of medium composition, temperature, method of inoculation and process of cultivation on growth of Aureobasidium pullulans. In the current work being conducted , cross-linked wheat straw were used as substrate. Aureobasidium pullulans was tested for the ability to produce hydroxylases, especially laccase and also for the ability to produce release - reducing sugars and proteins in basal media. Laccase oxidized a redox indicator ABTS ( 2,2' -azino-di (3-ethylbenzothiazoline- 6-sulfonic acid ) to form a blue - green cation radical.The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates. The method can be adapted for quantitative estimation by spectrophotometry. Aureobasidium pullulans were grown in different type of solid media, but less growth was observed in the course of liquid cultivations regardless of temperature. During the optimalization of the culture medium hydroxylase activity reached higher level in the presence of yeast Aureobasidium pulllulans in the glucose medium without wheat straw during submerged cultivation.

The present paper aims to study the influence of medium composition, temperature, method of inoculation and process of cultivation on growth of Aureobasidium pullulans. In the current work being conducted , cross-linked wheat straw were used as substrate. Aureobasidium pullulans was tested for the ability to produce hydroxylases, especially laccase and also for the ability to produce release - reducing sugars and proteins in basal media. Laccase oxidized a redox indicator ABTS ( 2,2' -azino-di (3-ethylbenzothiazoline- 6-sulfonic acid ) to form a blue - green cation radical.The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates. The method can be adapted for quantitative estimation by spectrophotometry. Aureobasidium pullulans were grown in different type of solid media, but less growth was observed in the course of liquid cultivations regardless of temperature. During the optimalization of the culture medium hydroxylase activity reached higher level in the presence of yeast Aureobasidium pulllulans in the glucose medium without wheat straw during submerged cultivation.

Keywords: Aureobasidium pullulans, wheat straw

Keywords: Aureobasidium pullulans, wheat straw

OMELKOVÁ, J.

2010

09.09.2008

1213-7103

CHEMICKE LISTY

102

15

Czech Republic

745

746

2