Publication result detail

Optimalization of Method for Quantification of Streptococcus Mutans to Dental Materials

OMELKOVÁ, J.

Original Title

Optimalization of Method for Quantification of Streptococcus Mutans to Dental Materials

English Title

Optimalization of Method for Quantification of Streptococcus Mutans to Dental Materials

Type

Peer-reviewed article not indexed in WoS or Scopus

Original Abstract

Microbial adhesion to the surface of composite resins and other restorative materials is an important parameter in the aetiology of dental caries. The aim of this study was to optimized the method for quantification of plaque deposition. The test surfaces used were a commercial microhybrid composite (Adoro, Ivoclar Vivadent), a commercial D3MA resin-based material (Advanced Dental Materials) and a commercial ceramic material (Ivoclar Vivadent) as reference material. The adhesion was tested with Streptococcus mutans. Microbial culture was inoculated into sterile test tubes in BHIB with tested specimens and incubated anaerobically for 17 hours. For the determination of bacterial adhesion the modified method of Biuret was used. Protein concentration was obtained spectrophotometrically at 540 nm.

English abstract

Microbial adhesion to the surface of composite resins and other restorative materials is an important parameter in the aetiology of dental caries. The aim of this study was to optimized the method for quantification of plaque deposition. The test surfaces used were a commercial microhybrid composite (Adoro, Ivoclar Vivadent), a commercial D3MA resin-based material (Advanced Dental Materials) and a commercial ceramic material (Ivoclar Vivadent) as reference material. The adhesion was tested with Streptococcus mutans. Microbial culture was inoculated into sterile test tubes in BHIB with tested specimens and incubated anaerobically for 17 hours. For the determination of bacterial adhesion the modified method of Biuret was used. Protein concentration was obtained spectrophotometrically at 540 nm.

Keywords

adhesion, dental materials

Key words in English

adhesion, dental materials

Authors

OMELKOVÁ, J.

RIV year

2010

Released

09.09.2008

ISBN

1213-7103

Periodical

CHEMICKE LISTY

Volume

102

Number

15

State

Czech Republic

Pages from

1152

Pages to

1154

Pages count

3

BibTex

@article{BUT47270,
  author="Jiřina {Omelková}",
  title="Optimalization of Method for Quantification of Streptococcus Mutans to Dental Materials",
  journal="CHEMICKE LISTY",
  year="2008",
  volume="102",
  number="15",
  pages="1152--1154",
  issn="0009-2770"
}