Publication result detail
Optimalization of Method for Quantification of Streptococcus Mutans to Dental Materials
OMELKOVÁ, J.
Original Title
Optimalization of Method for Quantification of Streptococcus Mutans to Dental Materials
English Title
Optimalization of Method for Quantification of Streptococcus Mutans to Dental Materials
Type
Peer-reviewed article not indexed in WoS or Scopus
Original Abstract
Microbial adhesion to the surface of composite resins and other restorative materials is an important parameter in the aetiology of dental caries. The aim of this study was to optimized the method for quantification of plaque deposition. The test surfaces used were a commercial microhybrid composite (Adoro, Ivoclar Vivadent), a commercial D3MA resin-based material (Advanced Dental Materials) and a commercial ceramic material (Ivoclar Vivadent) as reference material. The adhesion was tested with Streptococcus mutans. Microbial culture was inoculated into sterile test tubes in BHIB with tested specimens and incubated anaerobically for 17 hours. For the determination of bacterial adhesion the modified method of Biuret was used. Protein concentration was obtained spectrophotometrically at 540 nm.
English abstract
Microbial adhesion to the surface of composite resins and other restorative materials is an important parameter in the aetiology of dental caries. The aim of this study was to optimized the method for quantification of plaque deposition. The test surfaces used were a commercial microhybrid composite (Adoro, Ivoclar Vivadent), a commercial D3MA resin-based material (Advanced Dental Materials) and a commercial ceramic material (Ivoclar Vivadent) as reference material. The adhesion was tested with Streptococcus mutans. Microbial culture was inoculated into sterile test tubes in BHIB with tested specimens and incubated anaerobically for 17 hours. For the determination of bacterial adhesion the modified method of Biuret was used. Protein concentration was obtained spectrophotometrically at 540 nm.
Keywords
adhesion, dental materials
Key words in English
adhesion, dental materials
Authors
OMELKOVÁ, J.
RIV year
2010
Released
09.09.2008
ISBN
1213-7103
Periodical
CHEMICKE LISTY
Volume
102
Number
15
State
Czech Republic
Pages from
1152
Pages to
1154
Pages count
3
BibTex
@article{BUT47270,
author="Jiřina {Omelková}",
title="Optimalization of Method for Quantification of Streptococcus Mutans to Dental Materials",
journal="CHEMICKE LISTY",
year="2008",
volume="102",
number="15",
pages="1152--1154",
issn="0009-2770"
}