Publication result detail
Strategies for Enhancing Poly(3-hydroxybutyrate) Production in Selected Bacterial Strains
OBRUČA, S.; MELUŠOVÁ, S.; MÁROVÁ, I.; SVOBODA, Z.
Original Title
Strategies for Enhancing Poly(3-hydroxybutyrate) Production in Selected Bacterial Strains
English Title
Strategies for Enhancing Poly(3-hydroxybutyrate) Production in Selected Bacterial Strains
Type
Peer-reviewed article not indexed in WoS or Scopus
Original Abstract
Polyhydroxyalkanoates (PHAs) are a group of bacterial polyesters that have received much interest in recent times due to their biodegradable nature and mechanical properties which are very similar to traditional plastics from petrochemical routes. The most common PHA is poly(3-hydroxybutyrate) (PHB). Many bacterial strains use PHB as carbon and reducing power storage material. Bacterial strains usually accumulate PHB during exposition to stress condition. Typical strategy, which is widely used for enhancing of production, is limitation with nitrogen, phosphor or another element when excess amount of carbon source is present. The influences of selected stress factors on accumulation of PHB were tested in this work. Limitations with nitrogen and/or phosphor source were tested during growth of bacteria on synthetic as well as natural waste carbon source. Oxygen limitation was studied too. Finally, peroxide stress, osmotic stress, ethanol stress and UV radiation were studied as additional stress factors to nutrition starvation. Combined effect of exogenous stress could lead to increased accumulation of PHB in selected bacterial strains.
English abstract
Polyhydroxyalkanoates (PHAs) are a group of bacterial polyesters that have received much interest in recent times due to their biodegradable nature and mechanical properties which are very similar to traditional plastics from petrochemical routes. The most common PHA is poly(3-hydroxybutyrate) (PHB). Many bacterial strains use PHB as carbon and reducing power storage material. Bacterial strains usually accumulate PHB during exposition to stress condition. Typical strategy, which is widely used for enhancing of production, is limitation with nitrogen, phosphor or another element when excess amount of carbon source is present. The influences of selected stress factors on accumulation of PHB were tested in this work. Limitations with nitrogen and/or phosphor source were tested during growth of bacteria on synthetic as well as natural waste carbon source. Oxygen limitation was studied too. Finally, peroxide stress, osmotic stress, ethanol stress and UV radiation were studied as additional stress factors to nutrition starvation. Combined effect of exogenous stress could lead to increased accumulation of PHB in selected bacterial strains.
Keywords
poly(3-hydroxybutyrate), stress condition
Key words in English
poly(3-hydroxybutyrate), stress condition
Authors
OBRUČA, S.; MELUŠOVÁ, S.; MÁROVÁ, I.; SVOBODA, Z.
RIV year
2010
Released
09.09.2008
Publisher
CSCH
Location
Brno
ISBN
1213-7103
Periodical
CHEMICKE LISTY
Volume
102
Number
15
State
Czech Republic
Pages from
1255
Pages to
1256
Pages count
2
Full text in the Digital Library
BibTex
@article{BUT48296,
author="Stanislav {Obruča} and Soňa {Melušová} and Ivana {Márová} and Zdeněk {Svoboda}",
title="Strategies for Enhancing Poly(3-hydroxybutyrate) Production in Selected Bacterial Strains",
journal="CHEMICKE LISTY",
year="2008",
volume="102",
number="15",
pages="1255--1256",
issn="0009-2770"
}