Publication result detail
GROWTH CURVES OF MIXED THERMOPHILIC BACTERIA
BABÁK, L.; BURDYCHOVÁ, R.; DOHNAL, V.
Original Title
GROWTH CURVES OF MIXED THERMOPHILIC BACTERIA
English Title
GROWTH CURVES OF MIXED THERMOPHILIC BACTERIA
Type
Peer-reviewed article not indexed in WoS or Scopus
Original Abstract
The growth of mixed thermophilic bacteria culture (sludge of wastewater treatment Bystřice pod Hostýnem) was studied. Two different cultivation systems were used for monitoring and comparison of growth characteristics of above mentioned thermophilic culture. The first system consisted of batch cultivations in cuvettes with very little volumes (10-5 L) of cultivation media at 60 ?C. Samples were taken by autosampler in periodic intervals (24,4 min). Bacteria growth was there observed by means of HPLC with UV detection (600 nm). The second system was composed of batch cultivations in the laboratory fermentor BIOSTAT B ( B. Braun Biotech) at 60 ?C, too. The system was aerated (10 L.min-1) and stired by multiple turbo-stirrer (250 min-1). Samples were taken every two hours. Biomass concentration was detected by turbidimeter. Both cultivation ways were practised in several substrates with different C-sources (glucose, sacharose, lactose, maltose). Obtained growth curves were comparised. Furthermore, the biomass productivity values were determined.
English abstract
The growth of mixed thermophilic bacteria culture (sludge of wastewater treatment Bystřice pod Hostýnem) was studied. Two different cultivation systems were used for monitoring and comparison of growth characteristics of above mentioned thermophilic culture. The first system consisted of batch cultivations in cuvettes with very little volumes (10-5 L) of cultivation media at 60 ?C. Samples were taken by autosampler in periodic intervals (24,4 min). Bacteria growth was there observed by means of HPLC with UV detection (600 nm). The second system was composed of batch cultivations in the laboratory fermentor BIOSTAT B ( B. Braun Biotech) at 60 ?C, too. The system was aerated (10 L.min-1) and stired by multiple turbo-stirrer (250 min-1). Samples were taken every two hours. Biomass concentration was detected by turbidimeter. Both cultivation ways were practised in several substrates with different C-sources (glucose, sacharose, lactose, maltose). Obtained growth curves were comparised. Furthermore, the biomass productivity values were determined.
Keywords
thermophilic, growth curve, HPLC/UV, bioreactor
Key words in English
thermophilic, growth curve, HPLC/UV, bioreactor
Authors
BABÁK, L.; BURDYCHOVÁ, R.; DOHNAL, V.
RIV year
2012
Released
11.09.2008
Publisher
ČSCH
Location
Praha
ISBN
1803-2389
Periodical
Chemické listy (S)
Volume
102
Number
15
State
Czech Republic
Pages from
583
Pages to
584
Pages count
2
BibTex
@article{BUT48885,
author="Libor {Babák} and Radka {Burdychová} and Vlastimil {Dohnal}",
title="GROWTH CURVES OF MIXED THERMOPHILIC BACTERIA",
journal="Chemické listy (S)",
year="2008",
volume="102",
number="15",
pages="583--584",
issn="1803-2389"
}