Publication result detail
Oligogalacturonate hydrolase with unique substrate preference from the pulp of parsley roots
OMELKOVÁ, J.; FLODROVÁ, D.; STRATILOVÁ, E.
Original Title
Oligogalacturonate hydrolase with unique substrate preference from the pulp of parsley roots
English Title
Oligogalacturonate hydrolase with unique substrate preference from the pulp of parsley roots
Type
Peer-reviewed article not indexed in WoS or Scopus
Original Abstract
The main form of pectate hydrolases in the cell wall of parsley roots showed a unique substrate preference of a plant exopolygalacturonase because it clearly preferred the substrates with degree of polymerization about 10. This form was separated from the others, purified and characterized. Enzyme exhibited sharp pH optimum corresponding to pH 4.7, molecular mass 53.5 kDa, and isoelectric point 5.3. It was stable at 50A degrees C in 2-h assay and had optimum of temperature at 60A degrees C (activation energy being 37.0 kJ/mol). The interaction with concanavalin A indicated the glycosylation of enzyme. Substrates were cleaved from the non-reducing end.
English abstract
The main form of pectate hydrolases in the cell wall of parsley roots showed a unique substrate preference of a plant exopolygalacturonase because it clearly preferred the substrates with degree of polymerization about 10. This form was separated from the others, purified and characterized. Enzyme exhibited sharp pH optimum corresponding to pH 4.7, molecular mass 53.5 kDa, and isoelectric point 5.3. It was stable at 50A degrees C in 2-h assay and had optimum of temperature at 60A degrees C (activation energy being 37.0 kJ/mol). The interaction with concanavalin A indicated the glycosylation of enzyme. Substrates were cleaved from the non-reducing end.
Keywords
EXO-D-GALACTURONANASE; ASPERGILLUS-NIGER; GLYCOSIDE HYDROLASES; BIOCHEMICAL-CHARACTERIZATION; ENDOPOLYGALACTURONASE-I; CRYSTAL-STRUCTURE; EXOPOLYGALACTURONASE; POLYGALACTURONASE; CARROT; CHROMATOGRAPHY
Key words in English
EXO-D-GALACTURONANASE; ASPERGILLUS-NIGER; GLYCOSIDE HYDROLASES; BIOCHEMICAL-CHARACTERIZATION; ENDOPOLYGALACTURONASE-I; CRYSTAL-STRUCTURE; EXOPOLYGALACTURONASE; POLYGALACTURONASE; CARROT; CHROMATOGRAPHY
Authors
OMELKOVÁ, J.; FLODROVÁ, D.; STRATILOVÁ, E.
RIV year
2012
Released
02.04.2009
ISBN
0006-3088
Periodical
BIOLOGIA
Volume
2009 (64)
Number
2
State
Slovak Republic
Pages from
228
Pages to
234
Pages count
7
BibTex
@article{BUT48997,
author="Jiřina {Omelková} and Dana {Flodrová} and Eva {Stratilová}",
title="Oligogalacturonate hydrolase with unique substrate preference from the pulp of parsley roots",
journal="BIOLOGIA",
year="2009",
volume="2009 (64)",
number="2",
pages="228--234",
issn="0006-3088"
}