Publication result detail
Finishing the S.pombe genome deletion project. An update.
ČARNECKÁ, M.; MÁROVÁ, I.
Original Title
Finishing the S.pombe genome deletion project. An update.
English Title
Finishing the S.pombe genome deletion project. An update.
Type
Peer-reviewed article not indexed in WoS or Scopus
Original Abstract
The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. the collection of deletion mutants now covers 99% of the fission yeast open reading frames.
English abstract
The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. the collection of deletion mutants now covers 99% of the fission yeast open reading frames.
Keywords
fission yeast; gene deletion; genomics; essential genes; vectors
Key words in English
fission yeast; gene deletion; genomics; essential genes; vectors
Authors
ČARNECKÁ, M.; MÁROVÁ, I.
RIV year
2011
Released
28.07.2010
Publisher
LANDES BIOSCIENCE
Location
USA
ISBN
1538-4101
Periodical
CELL CYCLE
Volume
9
Number
12
State
United States of America
Pages from
2399
Pages to
2402
Pages count
4
BibTex
@article{BUT50225,
author="Martina {Čarnecká} and Ivana {Márová}",
title="Finishing the S.pombe genome deletion project. An update.",
journal="CELL CYCLE",
year="2010",
volume="9",
number="12",
pages="2399--2402",
issn="1538-4101"
}