Detail publikačního výsledku

Analysis of the noncaloric protein thaumatin by the micellar electrokinetic capillary chromatography

KRČMOVÁ, M.; VESPALCOVÁ, M.; LANGAUF, A.

Original Title

Analysis of the noncaloric protein thaumatin by the micellar electrokinetic capillary chromatography

English Title

Analysis of the noncaloric protein thaumatin by the micellar electrokinetic capillary chromatography

Type

Abstract

Original Abstract

Thaumatin is an alternative sweetener of plant origin. It is isolated from the tropical plant Thaumatococcus daniellii Benth.. Thaumatin is 3 000 - 5 000 times sweeter than sucrose. This sweet protein have two forms: - thaumatin I (22.2 kDa), - thaumatin II (22.3 kDa). Both of them contain 207 amino acids in the molecule. Beyond thaumatin I and thaumatin II contains the isolate from plant 3 minor molecules (thaumatin a, b, c) and another sweet proteins. Thaumatin is strong basic protein of pI = 11.7 - 12. This poster demonstrated the MECC separation applicability for the analysis of this sweet protein. The advantages of MECC method are its simplicity, cheapness and short analysing time.

English abstract

Thaumatin is an alternative sweetener of plant origin. It is isolated from the tropical plant Thaumatococcus daniellii Benth.. Thaumatin is 3 000 - 5 000 times sweeter than sucrose. This sweet protein have two forms: - thaumatin I (22.2 kDa), - thaumatin II (22.3 kDa). Both of them contain 207 amino acids in the molecule. Beyond thaumatin I and thaumatin II contains the isolate from plant 3 minor molecules (thaumatin a, b, c) and another sweet proteins. Thaumatin is strong basic protein of pI = 11.7 - 12. This poster demonstrated the MECC separation applicability for the analysis of this sweet protein. The advantages of MECC method are its simplicity, cheapness and short analysing time.

Keywords

sweet protein thaumatin; basic protein; MEKC; SDS;

Key words in English

sweet protein thaumatin; basic protein; MEKC; SDS;

Authors

KRČMOVÁ, M.; VESPALCOVÁ, M.; LANGAUF, A.

RIV year

2012

Released

18.10.2004

Publisher

Dechema

Location

Aachen

Book

Book of Abstracts

Pages from

94

Pages to

95

Pages count

2

Full text in the Digital Library

BibTex

@misc{BUT60295,
  author="Miroslava {Krčmová} and Milena {Vespalcová} and Aleš {Langauf}",
  title="Analysis of the noncaloric protein thaumatin by the micellar electrokinetic capillary chromatography",
  booktitle="Book of Abstracts",
  year="2004",
  pages="94--95",
  publisher="Dechema",
  address="Aachen",
  note="Abstract"
}