Detail publikačního výsledku
Nano- and microparticles for molecular diagnostic applications
RITTICH, B., TRACHTOVÁ, Š., ŠPANOVÁ, A., KAMAN, O., POLLERT E. HORÁK, D.
Original Title
Nano- and microparticles for molecular diagnostic applications
English Title
Nano- and microparticles for molecular diagnostic applications
Type
Abstract
Original Abstract
Research has been focused on the development of innovative magnetic nano- and micrometer-sized particles for specific applications in DNA analysis. Hybride nanoparticles of lanthanum manganese perovskites (La1-xSrxMnO3) cores stabilized in the water suspension by silica shell recently developed for medical applications were now tested for the use in DNA analysis. They were applied for the isolation of bacterial DNA from bacterial cell lysates using reversible adsorption in an aqueous phase system consisting of 16% PEG 6000 and 2 M sodium chloride. Thermosensitive monodisperse PNIPAAm microparticles were tested for reversible adsorption of DNA at different temperatures. The quality of isolated DNAs was tested using agarose gel electrophoresis and as a matrix in PCR.
English abstract
Research has been focused on the development of innovative magnetic nano- and micrometer-sized particles for specific applications in DNA analysis. Hybride nanoparticles of lanthanum manganese perovskites (La1-xSrxMnO3) cores stabilized in the water suspension by silica shell recently developed for medical applications were now tested for the use in DNA analysis. They were applied for the isolation of bacterial DNA from bacterial cell lysates using reversible adsorption in an aqueous phase system consisting of 16% PEG 6000 and 2 M sodium chloride. Thermosensitive monodisperse PNIPAAm microparticles were tested for reversible adsorption of DNA at different temperatures. The quality of isolated DNAs was tested using agarose gel electrophoresis and as a matrix in PCR.
Keywords
nanoparticles; thermosensitive microparticles; DNA separation; PCR
Key words in English
nanoparticles; thermosensitive microparticles; DNA separation; PCR
Authors
RITTICH, B., TRACHTOVÁ, Š., ŠPANOVÁ, A., KAMAN, O., POLLERT E. HORÁK, D.
RIV year
2011
Released
05.09.2010
Publisher
Dechema
Location
Bologna
Pages from
B2004
Pages to
B2004
Pages count
1
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