R&D result detail

Original Title

Determination of sulphonamides in wastewater

English Title

Determination of sulphonamides in wastewater

Type

Audiovisual work

Original Abstract

Sulphonamides are widely used antibiotics in human and veterinary medicine. These compounds have been shown to block biological processes in wastewater treatment plants. Furthermore, bacterial resistence against them has been proven to establish easily. The focus of this research was to developed separation and identification technique for sulphonamides in wastewater. Sulphonamides were concentrated by l/l extraction using ethyl acetate. The HPLC column ZORBAX Eclipse XDB-C18 (150mm x 4.6mm) was used and elution was carried out using gradient mode. As mobile phase was used acetonitrile and 0,1% formic acid in purified water. Sulphonamides were detected using UV absorbance at 270 nm. Average recovery obtained for sulfonamides was 84.1 6.8 %. Limit of quatification (LOQ) for sulfathiazol was 0.5 ng.ml-1 and limit of detection was 0.2 ng.ml-1. The optimized method was used for real samples collected from wastewater treatment plant in University of Veterinary and Pharmaceutical Sciences Brno campus.

English abstract

Sulphonamides are widely used antibiotics in human and veterinary medicine. These compounds have been shown to block biological processes in wastewater treatment plants. Furthermore, bacterial resistence against them has been proven to establish easily. The focus of this research was to developed separation and identification technique for sulphonamides in wastewater. Sulphonamides were concentrated by l/l extraction using ethyl acetate. The HPLC column ZORBAX Eclipse XDB-C18 (150mm x 4.6mm) was used and elution was carried out using gradient mode. As mobile phase was used acetonitrile and 0,1% formic acid in purified water. Sulphonamides were detected using UV absorbance at 270 nm. Average recovery obtained for sulfonamides was 84.1 6.8 %. Limit of quatification (LOQ) for sulfathiazol was 0.5 ng.ml-1 and limit of detection was 0.2 ng.ml-1. The optimized method was used for real samples collected from wastewater treatment plant in University of Veterinary and Pharmaceutical Sciences Brno campus.

Keywords

sulphonamides; wastewater; HPLC; DAD

Key words in English

sulphonamides; wastewater; HPLC; DAD

Authors

VÍTEČKOVÁ, H.; MRAVCOVÁ, L.; VÁVROVÁ, M.

Released

04.12.2006

Publisher

Brno University of Technology, Faculty of Chemistry

Location

Brno

ISBN

80-214-3320-5

Book

the book of abstract

Edition

1

Pages from

224

Pages to

224

Pages count

1

URL

http://www.fch.vutbr.cz

Full text in the Digital Library

http://hdl.handle.net/

BibTex

@misc{BUT63484,
  author="Hana {Lisá} and Ludmila {Mravcová} and Milada {Vávrová}",
  title="Determination of sulphonamides in wastewater",
  booktitle="the book of abstract",
  year="2006",
  series="1",
  edition="1",
  pages="224--224",
  publisher="Brno University of Technology, Faculty of Chemistry",
  address="Brno",
  isbn="80-214-3320-5",
  url="http://www.fch.vutbr.cz",
  note="Audiovisual work"
}